| 000 | 00000nam c2200205 c 4500 | |
| 001 | 000046146599 | |
| 005 | 20230413110110 | |
| 007 | ta | |
| 008 | 210102s2021 ulkad bmAC 000c eng | |
| 040 | ▼a 211009 ▼c 211009 ▼d 211009 | |
| 085 | 0 | ▼a 0510 ▼2 KDCP |
| 090 | ▼a 0510 ▼b 6D5 ▼c 1235 | |
| 100 | 1 | ▼a 김리나, ▼g 金利娜 |
| 245 | 1 0 | ▼a Effect of protein surface hydrophobicity on protein adsorption and desorption / ▼d Kim Li Na |
| 260 | ▼a Seoul : ▼b Graduate School, Korea University, ▼c 2021 | |
| 300 | ▼a 48장 : ▼b 삽화, 도표 ; ▼c 26 cm | |
| 500 | ▼a 지도교수: 김중배 | |
| 502 | 0 | ▼a 학위논문(석사)-- ▼b 고려대학교 대학원: ▼c 화공생명공학과, ▼d 2021. 2 |
| 504 | ▼a 참고문헌: 장 43-48 | |
| 530 | ▼a PDF 파일로도 이용가능; ▼c Requires PDF file reader(application/pdf) | |
| 653 | ▼a Protein surface hydrophobicity ▼a Mesoporous materials ▼a Protein adsorption and desorption | |
| 776 | 0 | ▼t Effect of Protein Surface Hydrophobicity on Protein Adsorption and Desorption ▼w (DCOLL211009)000000235826 |
| 900 | 1 0 | ▼a Kim, Li-na, ▼e 저 |
| 900 | 1 0 | ▼a 김중배, ▼g 金重培, ▼e 지도교수 |
| 945 | ▼a ITMT |
전자정보
소장정보
| No. | 소장처 | 청구기호 | 등록번호 | 도서상태 | 반납예정일 | 예약 | 서비스 |
|---|---|---|---|---|---|---|---|
| No. 1 | 소장처 과학도서관/학위논문서고/ | 청구기호 0510 6D5 1235 | 등록번호 123069784 | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
| No. 2 | 소장처 과학도서관/학위논문서고/ | 청구기호 0510 6D5 1235 | 등록번호 123069785 | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
컨텐츠정보
초록
Adsorption and desorption behavior of protein molecules with nanostructured materials plays a key role in a variety of fields. Though the mechanism remains unclear, protein surface properties assigned by a variety of amino acids influence the surface interactions between proteins and nanomaterials. In this study, an investigation for surface interaction between protein molecules and porous nanomaterials was conducted via correlating their surface hydrophobicity with their adsorption and desorption behaviors. Based on the accessible surface hydrophobicity calculation and quantitative protein tracing experiments, the surface hydrophobicity of proteins showed a significant influence on the adsorption and desorption behavior. Further, the vinyl-functionalization of hydrophilic lysine groups on the protein surface resulted in an increased affinity between protein and porous nanomaterials. This work provides a quantitative demonstration for the contribution of surface hydrophobicity to the protein adsorption and desorption and proof of principle for controlling protein adsorption and desorption behavior via surface hydrophobicity modulation.
목차
1. Introduction - 1 - 1.1. Crowding and Confinement Effects of Proteins - 1 - 1.2. Crowding and Confinement Effects in Mesoporous Materials - 2 - 1.3. Adsorption and Desorption of Proteins in and from Nanomaterials - 4 - 1.4. Protein Surface Analysis - 6 - 1.5. Objective - 7 - 2. Experimental Section - 10 - 2.1. Materials - 10 - 2.2. Preparation of Mesoporous Silica and Mesoporous Carbon - 11 - 2.3. Determination of Protein Properties - 13 - 2.3.1. Hydrophobic Surface Area Estimation - 13 - 2.3.2. Protein Structures - 14 - 2.3.3. Characterization of Protein Surface Charge and Their Volume - 14 - 2.4. Protein Adsorption and Desorption - 18 - 2.4.1. Measurement of Protein Loading - 18 - 2.4.2. Vinyl Group Functionalization of Lysine Residue on Protein Surface - 19 - 2.4.3. Activity Measurements of Released GOx - 19 - 3. Results and Discussion - 20 - 3.1. Determination of Proteins Properties - 20 - 3.2. Correlation between Protein Properties and Desorption - 27 - 3.3. Effect of Hydrophobic Modulation - 29 - 3.4. Control of Protein Retention in Mesoporous Material via Different Proportions of Vinyl-Functionalized GOx - 39 - 4. Conclusions - 42 -
