Improvement of hexanoic acid production by Clostridium sp. JS66 through overexpressing thiolase and liquid-liquid extraction during mixotrophic fermentation
| 000 | 00000nam c2200205 c 4500 | |
| 001 | 000046146573 | |
| 005 | 20230718154437 | |
| 007 | ta | |
| 008 | 201230s2021 ulkad bmAC 000c eng | |
| 040 | ▼a 211009 ▼c 211009 ▼d 211009 | |
| 041 | 0 | ▼a eng ▼b kor |
| 085 | 0 | ▼a 0510 ▼2 KDCP |
| 090 | ▼a 0510 ▼b 6D5 ▼c 1234 | |
| 100 | 1 | ▼a 최도윤, ▼g 崔度尹 |
| 245 | 1 0 | ▼a Improvement of hexanoic acid production by Clostridium sp. JS66 through overexpressing thiolase and liquid-liquid extraction during mixotrophic fermentation / ▼d Doyoun Choi |
| 260 | ▼a Seoul : ▼b Graduate School, Korea University, ▼c 2021 | |
| 300 | ▼a iii, 64장 : ▼b 삽화, 도표 ; ▼c 26 cm | |
| 500 | ▼a 지도교수: 오민규 | |
| 500 | ▼a 지도교수: 엄영순 | |
| 502 | 0 | ▼a 학위논문(석사)-- ▼b 고려대학교 대학원, ▼c 화공생명공학과, ▼d 2021. 2 |
| 504 | ▼a 참고문헌: 장 60-63 | |
| 530 | ▼a PDF 파일로도 이용가능; ▼c Requires PDF file reader(application/pdf) | |
| 653 | ▼a Thiolase ▼a Hexanoic acid ▼a Liquid-liquid extraction ▼a Mixotrophic fermentation ▼a Clostridium | |
| 776 | 0 | ▼t Improvement of Hexanoic Acid Production by Clostridium sp. JS66 through Overexpressing Thiolase and Liquid-liquid Extraction during Mixotrophic Fermentation ▼w (DCOLL211009)000000235836 |
| 900 | 1 0 | ▼a Choi, Do-youn, ▼e 저 |
| 900 | 1 0 | ▼a 오민규, ▼g 吳敏圭, ▼d 1969-, ▼e 지도교수 ▼0 AUTH(211009)153483 |
| 900 | 1 0 | ▼a 엄영순, ▼e 지도교수 |
| 945 | ▼a ITMT |
Electronic Information
| No. | Title | Service |
|---|---|---|
| 1 | Improvement of hexanoic acid production by Clostridium sp. JS66 through overexpressing thiolase and liquid-liquid extraction during mixotrophic fermentation (3회 열람) |
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| No. 1 | Location Science & Engineering Library/Stacks(Thesis)/ | Call Number 0510 6D5 1234 | Accession No. 123069764 | Availability Available | Due Date | Make a Reservation | Service |
| No. 2 | Location Science & Engineering Library/Stacks(Thesis)/ | Call Number 0510 6D5 1234 | Accession No. 123069765 | Availability Available | Due Date | Make a Reservation | Service |
Contents information
Abstract
Synthesis gas (syngas), a mixture of H2, CO and CO2, can be produced not only from natural gas and coal but also from biomass, a renewable feedstock. To utilize syngas as liquid fuel, a conversion process via Fischer–Tropsch (FT) synthesis, a serial of chemical reactions, has been widely employed. However, it is inefficient in terms of energy due to a high pressure (20-40 bar) and temperature (200-360 °C). For this reason, biocatalystic gas fermentation using acetogen has been focused on producing sustainable biofuels in an environmental friendly way. In this study, to enhance the production of hexanoic acid (HA) by Clostridium sp. JS66 (JS66) producing HA from syngas, three strategies were implemented: 1) Homologous overexpression of thiolase (THL), 2) Liquid-liquid extraction (LLE), and 3) Mixotrophic fermentation. THL (CDS_416) in JS66 was identified to be essential enzyme for the chain elongation up to six-carbon and the genetically engineered JS66 strain by homologous overexpressing THL (CDS_416) was obtained. In the mixotrophic extractive fermentation using both syngas and glucose as carbon sources with 10% trioctylphosphine oxide (TOPO) in dodecane, JS66 strain overexpressing THL showed higher titer (8.3 g/L) and yield (0.44 HA C mol / substrate C mol) than the wild type by 15.3% and 10.0%, respectively. Furthermore, when acetate and butyrate were supplied as electron acceptors, HA titer and yield were produced 12.2 g/L and 0.70 (HA c mol / substrate c mol) under conditions 4 g/L sodium acetate and both of 4 g/L sodium acetate and 4 g/L sodium butyrate, respectively. It increased by 47.0% and 59.1% compared to non-condition in mutant. These results are valuable in terms of improving renewable biofuel (HA) production via utilizing glucose, one of the most abundant and affordable sugar, and sustainable syngas in single clostridia.
Table of Contents
ABSTRACT 1 1. Introduction 3 2. Materials and methods 9 2.1 Strains, medium and culture conditions 9 2.1.1 Strains 9 2.1.2 Medium and culture conditions 11 2.2 Construction of plasmids for overexpression Thiolase (THL) 14 2.3 Electro-transformation of clostridia 18 2.4 Identification of THL 20 2.5 Liquid-liquid extraction (LLE) 22 2.6 Fed-batch fermentation 24 3. Results 26 3.1 Identification of three paralogous THL in JS66 26 3.2 The effect of Liquid-liquid extraction(LLE) 29 3.3 The cultivation of THL (CDS_416) homologous overexpression strain 34 3.4 The effect of supplying acetate and butyrate in JS66 overexpressing THL (CDS_416) during mixotrophic fermentation 38 3.4.1 The effect of supplying acetate 38 3.4.2 The effect of supplying butyrate 43 3.4.3 The effect of supplying both of acetate and butyrate 47 4. Discussion 50 5. Reference 60 6. Abstract in Korean 64
