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Neural reprogramming : methods and protocols

Neural reprogramming : methods and protocols

Material type
단행본
Personal Author
Ahlenius, Henrik.
Title Statement
Neural reprogramming : methods and protocols / edited by Henrik Ahlenius.
Publication, Distribution, etc
New York :   Humana Press,   c2021.  
Physical Medium
xi, 263 p. : ill. (some col.) ; 27 cm.
Series Statement
Springer protocols
ISBN
9781071616000
Content Notes
Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts -- Direct In Vitro Reprogramming of Astrocytes into Induced Neurons -- Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9 -- Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules -- Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons -- Direct Conversion of Human Fibroblasts to Induced Neurons -- Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts -- Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs) -- Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors -- Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons -- Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression -- Transcriptional Profiling During Neural Conversion -- Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo -- Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods -- Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions -- CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders -- Derivation of Adult Human Cortical Organotypic Slice Cultures for Co-Culture with Reprogrammed Neuronal Cells.
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Neural circuitry --Laboratory manuals. Stem cells --Laboratory manuals. Neural circuitry.
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008 211014s2021 nyua b 001 0 eng d
020 ▼a 9781071616000
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 612.82 ▼2 23
084 ▼a 612.82 ▼2 DDCK
090 ▼a 612.82 ▼b N49492
245 0 0 ▼a Neural reprogramming : ▼b methods and protocols / ▼c edited by Henrik Ahlenius.
260 ▼a New York : ▼b Humana Press, ▼c c2021.
300 ▼a xi, 263 p. : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Springer protocols
490 1 ▼a Methods in molecular biology, ▼x 1940-6029 ; ▼v Volume 2352
504 ▼a Includes bibliographical references and index.
505 0 ▼a Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts -- Direct In Vitro Reprogramming of Astrocytes into Induced Neurons -- Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9 -- Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules -- Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons -- Direct Conversion of Human Fibroblasts to Induced Neurons -- Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts -- Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs) -- Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors -- Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons -- Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression -- Transcriptional Profiling During Neural Conversion -- Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo -- Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods -- Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions -- CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders -- Derivation of Adult Human Cortical Organotypic Slice Cultures for Co-Culture with Reprogrammed Neuronal Cells.
650 0 ▼a Neural circuitry ▼v Laboratory manuals.
650 0 ▼a Stem cells ▼v Laboratory manuals.
650 0 ▼a Neural circuitry.
700 1 ▼a Ahlenius, Henrik.
830 0 ▼a Springer protocols.
830 0 ▼a Methods in molecular biology ; ▼v v. 2352.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 612.82 N49492 Accession No. 521006132 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

1. Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts



Juan M. Adrian-Segarra, Bettina Weigel, and Moritz Mall







2. Direct In Vitro Reprogramming of Astrocytes into Induced Neurons



Nesrin Sharif, Filippo Calzolari, and Benedikt Berninger







3. Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9



Boning Qiu, Ruben J. de Vries, and Massimiliano Caiazzo







4. Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules



E Tian, Mingzi Zhang, and Yanhong Shi







5. Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons



Amel Falco, Rocio Bartolome-Cabrero, and Sergio Gascon







6. Direct Conversion of Human Fibroblasts to Induced Neurons



Lucia Zhou-Yang, Sophie Eichhorner, Lukas Karbacher, Lena Boehnke, Larissa Traxler, and Jerome Mertens







7. Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts



Emilie M. Legault and Janelle Drouin-Ouellet







8. Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs)



Ruiqi Hu, Xiaoting Zhu, and Nan Yang







9. Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors



Keiko Imamura, Jitsutaro Kawaguchi, Tsugumine Shu, and Haruhisa Inoue







10. Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons



Ella Quist, Henrik Ahlenius, and Isaac Canals







11. Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression



Katrien Neyrinck and Juan A. Garcia-Leon







12. Transcriptional Profiling During Neural Conversion



Yohannes Afeworki, Hannah Wollenzien, and Michael S. Kareta







13. Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo



Srisaiyini Kidnapillai and Daniella Rylander Ottosson







14. Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods



Andreas Heuer







15. Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions



Juan M. Adrian-Segarra, Bettina Weigel, and Moritz Mall







16. CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders



Isaac Canals and Henrik Ahlenius







17. Derivation of Adult Human Cortical Organotypic Slice Cultures for Co-Culture with Reprogrammed Neuronal Cells



Giedre Kvist

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