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Pluripotent stem-cell derived cardiomyocytes

Pluripotent stem-cell derived cardiomyocytes

자료유형
단행본
서명 / 저자사항
Pluripotent stem-cell derived cardiomyocytes / edited by Yoshinori Yoshida.
발행사항
New York, NY :   Humana Press,   c2021.  
형태사항
xiv, 304 p. : ill. (chiefly col.) ; 27 cm.
총서사항
Methods in molecular biology,1064-3745 ; 2320
ISBN
9781071614839
서지주기
Includes bibliographical references and index.
일반주제명
Embryonic stem cells --Laboratory manuals. Cardiovascular system --Diseases --Laboratory manuals.
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001 000046095584
005 20211012094402
008 211012s2021 nyua b 001 0 eng d
020 ▼a 9781071614839
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 616.02774 ▼2 23
084 ▼a 616.02774 ▼2 DDCK
090 ▼a 616.02774 ▼b P7372
245 0 0 ▼a Pluripotent stem-cell derived cardiomyocytes / ▼c edited by Yoshinori Yoshida.
260 ▼a New York, NY : ▼b Humana Press, ▼c c2021.
300 ▼a xiv, 304 p. : ▼b ill. (chiefly col.) ; ▼c 27 cm.
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 2320
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
650 0 ▼a Embryonic stem cells ▼v Laboratory manuals.
650 0 ▼a Cardiovascular system ▼x Diseases ▼v Laboratory manuals.
830 0 ▼a Methods in molecular biology ; ▼v 2320.
830 0 ▼a Springer protocols.
945 ▼a KLPA

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 616.02774 P7372 등록번호 521006123 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

목차

Part I: Overview



1. Making Cardiomyocytes from Pluripotent Stem Cells



Peter Karaginius and Yoshinori Yoshida







Part II: Generation of Pluripotent Stem Cell-Derived Cardiomyocytes and Cardiac Tissues



2. A method for Large-Scale Cardiac Differentiation, Purification, and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells



Yuika Morita, Shugo Tohyama, Jun Fujita, and Keiichi Fukuda







3. Large-scale Differentiation of Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture



Nagako Sougawa, Shigeru Miyagawa, and Yoshiki Sawa







4. Efficient Method to Dissociate Induced Pluripotent Stem Cell-derived Cardiomyocyte Aggregates into Single Cells



Emiko Ito, Shigeru Miyagawa, Yoshinori Yoshida, and Yoshiki Sawa







5. Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells using miRNA switches



Kenji Miki, Hirohide Saito, and Yoshinori Yoshida







6. Fabrication of Cardiac Constructs using bio-3D Printer



Kenichi Arai, Daiki Murata, Shoko Takao, and Koichi Nakayama







7. Fabrication of Thick and Anisotropic Cardiac Tissue on Nanofibrous Substrate for Repairing Infarcted Myocardium



Junjun Li, Li Liu, Itsunari Minami, Shigeru Miyagawa, and Yoshiki Sawa







8. Construction of Three-Dimensional Cardiac Tissues Using Layer by Layer Method



Maki Takeda, Shigeru Miyagawa, Mitsuru Akashi, and Yoshiki Sawa







9. Generation of Cylindrical Engineered Cardiac Tissues from human iPS Cell-derived Cardiovascular Cell Lineages



Hidetoshi Masumoto







Part III: Physiological Mesurements using Pluripotent Stem Cell-Derived Cardiomyocytes







10. Protocol for Morphological and Functional Phenotype Analysis of hiPS-derived Cardiomyocytes



Jun LI and Jong-Kook LEE







11. Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies



Tadashi Takaki and Yoshinori Yoshida







12. Multi-Electrode Array Assays Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes



Daisuke Yoshinaga, Yimin Wuriyanghai, and Takeru Makiyama







13. Electrophysiological Analysis of hiPSC-derived Cardiomyocytes Using a Patch-Clamp Technique



Yuta Yamamoto, Sayako Hirose, Yimin Wuriyanghai, Daisuke Yoshinaga, and Takeru Makiyama







14. Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human Induced Pluripotent Stem Cells



Misato Koakutsu, Tadashi Takaki, Kenji Miki, and Yoshinori Yoshida







15. Assessment of Contractility in Human iPS Cell-Derived Cardiomyocytes Using Motion Vector Analysis



Yasunari Kanda, Ayano Satsuka, Sayo Hayashi, Mihoko Hagiwara-Nagasawa, and Atsushi Sugiyama







16. Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells



Daisuke Sasaki, Katsuhisa Matsuura, and Tatsuya Shimizu







17. A Method for Contraction Force Measurement of iPSC Derived Engineered Cardiac Tissues



Yuya Fujiwara, Kohei Deguchi, Kenji Miki, Tomoyuki Nishimoto, and Yoshinori Yoshida







Part IV: Transcriptome and Bioinfomatics Analysis



18. Single-cardiomyocyte RNA Sequencing to Dissect the Molecular Pathophysiology of the Heart



Manami Kotoh, Seitaro Nomura, Shintaro Yamada,Hiroyuki Aburatani, and Issei Komuro







19. RNA-sequencing Analysis of Differentially Expressed Genes in Human iPSC-derived Cardiomyocytes



Chikako Okubo, Megumi Narita, Takuya Yamamoto, and Yoshinori Yoshida







20. Analysis of Transcriptional Profiling of Chamber-specific Human Cardiac Myocytes Derived from Pluripotent Stem Cells



Antonio Lucena-Cacace and Yoshinori Yoshida







Part V: Gene Editing and CRISPR Technology for Pluripotent Stem Cells



21. Genome Editing in Human Induced Pluripotent Stem Cells (hiPSCs)



Shuichiro Higo, Shungo Hikoso, Shigeru Miyagawa, and Yasushi Sakata







22. Generation of Efficient Knock-in Mouse and Human Pluripotent Stem Cells Using CRISPR-Cas9



Tatsuya Anzai, Hiromasa Hara, Chanthra Nawin, Taketaro Sadahiro, Masaki Ieda, Yutaka Hanazono, and Hideki Uosaki







23. CRISPRi/a Screening with Human iPSCs



Masataka Nishiga, Lei S. Qi, and Joseph C. Wu







Part VI: Transplantation of Pluripotent Stem Cell-Derived Cardiomyocytes



24. Transplantation of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes in a Mouse Myocardial Infarction Model



Takeshi Hatani and Yoshinori Yoshida







25. Transplantation of Pluripotent Stem Cell-derived Cardiomyocytes into a Myocardial Infarction Model of Cynomolgus Monkey



Hideki Kobayashi, Hajime Ichimura, Noburou Ohashi, and Yuji Shiba

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