Part I: Design and Preparation of Biological Based Nanopores
1. Preparation of Fragaceatoxin C (FraC) Nanopores
Natalie Lisa Mutter, Gang Huang, Nieck van der Heide, Florian Leonardus Rudolfus Lucas, Nicole Stephanie Galenkamp, Giovanni Maglia, and Carsten Wloka
2. Preparation of Cytolysin A (ClyA) Nanopores
Nicole Stephanie Galenkamp, Veerle Van Meervelt, Natalie Lisa Mutter, Nieck van der Heide, Carsten Wloka, and Giovanni Maglia
3. Building Synthetic Transmembrane Peptide Pores
Kozhinjampara R. Mahendran
4. Design and Assembly of Membrane-Spanning DNA Nanopores
Kerstin Goepfrich and Ulrich F. Keyser
Part II: Single Molecule Detection and Analysis of Protein Analytes
5. Determining the Orientation of Porins in Planar Lipid Bilayers
Sandra A Ionescu, Sejeong Lee, and Hagan Bayley
6. Revelation of Function and Inhibition of Wza through Single Channel Studies
Lingbing Kong
7. Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore
Monifa A.V. Fahie, Bib Yang, Christina M. Chisholm, and Min Chen
8. Nanopore Enzymology to Study Protein Kinases and their Inhibition by Small Molecules
Leon Harrington, Leila T. Alexander, Stefan Knapp, and Hagan Bayley
9. A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore
Monifa A.V. Fahie, Bach Pham, Fanjun Li, and Min Chen
10. Oligonucleotide-Directed Protein Threading through a Rigid Nanopore
Garbine Celaya and David Rodriguez Larrea
11. Unfolding and Translocation of Proteins through an Alpha-Hemolysin Nanopore by ClpXP
Jeff Nivala, Logan Mulroney, Qing Luan, Robin Abu-Shumays, and Mark Akeson
Part III: Computational Analysis of Nanopore Behavior
12. Simulation of pH-Dependent, Loop-Based Membrane Protein Gating Using Pretzel
Alan Perez-Rathke, Monifa A.V. Fahie, Christina M. Chisholm, Min Chen, and Jie Liang
13. Free Energy Minimization for Vesicle Translocation through a Narrow Pore
Hamid R. Shojaei, Ahad Khaleghi Ardabili, and Murrugappan Muthukumar
Part IV: Droplet Interface Bilayer System
14. Single Ion-Channel Analysis in Droplet Interface Bilayer
Arash Manafirad
15. Continuous and Rapid Solution Exchange in a Lipid Bilayer Perfusion System Based on Droplet-Interface Bilayer
En-Hsin Lee
16. Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip
Xin Li and Min Chen
