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Human cytomegaloviruses : methods and protocols / 2nd ed

Human cytomegaloviruses : methods and protocols / 2nd ed

Material type
Personal Author
Yurochko, Andrew D.
Title Statement
Human cytomegaloviruses : methods and protocols / edited by Andrew D. Yurochko.
2nd ed.
Publication, Distribution, etc
New York :   Humana Press,   c2021.  
Physical Medium
xi, 468 p. : ill. (some col.) ; 26 cm.
Series Statement
Methods in molecular biology,1064-3745 ; 2244
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
000 00000nam u2200205 a 4500
001 000046092256
005 20210913163651
008 210913s2021 nyua 001 0 eng d
020 ▼a 9781071611104
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 616/.0194 ▼2 23
084 ▼a 616.0194 ▼2 DDCK
090 ▼a 616.0194 ▼b H9182-2
245 0 0 ▼a Human cytomegaloviruses : ▼b methods and protocols / ▼c edited by Andrew D. Yurochko.
250 ▼a 2nd ed.
260 ▼a New York : ▼b Humana Press, ▼c c2021.
300 ▼a xi, 468 p. : ▼b ill. (some col.) ; ▼c 26 cm.
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 2244
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
650 2 ▼a Cytomegalovirus.
700 1 ▼a Yurochko, Andrew D.
830 0 ▼a Methods in molecular biology ; ▼v 2244.
830 0 ▼a Springer protocols.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 616.0194 H9182-2 Accession No. 521006103 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

1. Overview of Human Cytomegalovirus Pathogenesis

Heather L. Fulkerson, Maciej T. Nogalski, Donna Collins-McMillen, and Andrew D. Yurochko

2. Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies

Giada Frascaroli and Christian Sinzger

3. Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection

Elizabeth A. Fortunato

4. The Use of Primary Human Cells to Analyze Human Cytomegalovirus Biology

Emma Poole, Ian Groves, Sarah Jackson, Mark Wills, and John Sinclair

5. Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation

Megan Peppenelli, Jason Buehler, and Felicia Goodrum

6. Collection and Isolation of CD14+ Primary Human Monocytes via Dual Density Centrifugation as a Model System to Study Human Cytomegalovirus Infection and Pathogenesis

Bailey S. Mosher, Heather Fulkerson, and Andrew D. Yurochko

7. Stable and Inducible Gene Knockdown in Primary Human Fibroblasts: A Versatile Tool to Study the Role of Human Cytomegalovirus Host Cell Factors

Anne-Charlotte Stilp, Patrick Koenig, Myriam Scherer, and Thomas Stamminger

8. Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis

M. Zeeshan Chaudhry, Martin Messerle, and Luka Cicin-Sain

9. Methods for Studying the Function of Cytomegalovirus GPCRs

Christine M. O''Connor and William E. Miller

10. Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions

Jamil Mahmud and Gary C. Chan

11. Antibody-Independent Quantification of Cytomegalovirus Virion Protein Incorporation Using HiBiT

Iris K.A. Jones and Daniel N. Streblow

12. Use of a Phosphoproteomic Screen to Profile Early Changes during HCMV Infection of Human Monocytes

Liudmila S. Chesnokova and Andrew D. Yurochko

13. A Generally Applicable CRISPR/Cas9 Screening Technique to Identify Host Genes Required for Virus Infection as Applied to Human Cytomegalovirus (HCMV) Infection of Epithelial Cells

Xiaofei E and Timothy F. Kowalik

14. Quantitative Electron Microscopy to Study HCMV Morphogenesis

Clarissa Read, Paul Walther, and Jens von Einem

15. Detection of Cytomegalovirus Interleukin 10 (cmvIL-10) by Enzyme-Linked Immunosorbent Assay (ELISA)

Vivian P. Young, Margarette C. Mariano, Lionel Faure, and Juliet V. Spencer

16. Techniques for Characterizing Cytomegalovirus-Encoded miRNAs

Nicole L. Diggins, Lindsey B. Crawford, Hillary M. Struthers, Lauren M. Hook, Igor Landais, Rebecca L. Skalsky, and Meaghan H. Hancock

17. Development of a huBLT Mouse Model to Study HCMV Latency, Reactivation, and Immune Response

Lindsey B. Crawford and Patrizia Caposio

18. Rodent Models of Congenital Cytomegalovirus Infection

Berislav Lisnic, Jelena Tomac, Djurdjica Cekinovic, Stipan Jonjic, and Vanda Juranic Lisnic

19. Recent Approaches and Strategies in the Generation of Anti-Human Cytomegalovirus Vaccines

Suresh B. Boppana and William J. Britt

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