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Human cytomegaloviruses : methods and protocols / 2nd ed

Human cytomegaloviruses : methods and protocols / 2nd ed

Material type
단행본
Personal Author
Yurochko, Andrew D.
Title Statement
Human cytomegaloviruses : methods and protocols / edited by Andrew D. Yurochko.
판사항
2nd ed.
Publication, Distribution, etc
New York :   Humana Press,   c2021.  
Physical Medium
xi, 468 p. : ill. (some col.) ; 26 cm.
Series Statement
Methods in molecular biology,1064-3745 ; 2244
ISBN
9781071611104
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Cytomegalovirus.
000 00000nam u2200205 a 4500
001 000046092256
005 20210913163651
008 210913s2021 nyua 001 0 eng d
020 ▼a 9781071611104
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 616/.0194 ▼2 23
084 ▼a 616.0194 ▼2 DDCK
090 ▼a 616.0194 ▼b H9182-2
245 0 0 ▼a Human cytomegaloviruses : ▼b methods and protocols / ▼c edited by Andrew D. Yurochko.
250 ▼a 2nd ed.
260 ▼a New York : ▼b Humana Press, ▼c c2021.
300 ▼a xi, 468 p. : ▼b ill. (some col.) ; ▼c 26 cm.
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 2244
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
650 2 ▼a Cytomegalovirus.
700 1 ▼a Yurochko, Andrew D.
830 0 ▼a Methods in molecular biology ; ▼v 2244.
830 0 ▼a Springer protocols.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 616.0194 H9182-2 Accession No. 521006103 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

1. Overview of Human Cytomegalovirus Pathogenesis



Heather L. Fulkerson, Maciej T. Nogalski, Donna Collins-McMillen, and Andrew D. Yurochko







2. Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies



Giada Frascaroli and Christian Sinzger







3. Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection



Elizabeth A. Fortunato







4. The Use of Primary Human Cells to Analyze Human Cytomegalovirus Biology



Emma Poole, Ian Groves, Sarah Jackson, Mark Wills, and John Sinclair







5. Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation



Megan Peppenelli, Jason Buehler, and Felicia Goodrum







6. Collection and Isolation of CD14+ Primary Human Monocytes via Dual Density Centrifugation as a Model System to Study Human Cytomegalovirus Infection and Pathogenesis



Bailey S. Mosher, Heather Fulkerson, and Andrew D. Yurochko







7. Stable and Inducible Gene Knockdown in Primary Human Fibroblasts: A Versatile Tool to Study the Role of Human Cytomegalovirus Host Cell Factors



Anne-Charlotte Stilp, Patrick Koenig, Myriam Scherer, and Thomas Stamminger







8. Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis



M. Zeeshan Chaudhry, Martin Messerle, and Luka Cicin-Sain







9. Methods for Studying the Function of Cytomegalovirus GPCRs



Christine M. O''Connor and William E. Miller







10. Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions



Jamil Mahmud and Gary C. Chan







11. Antibody-Independent Quantification of Cytomegalovirus Virion Protein Incorporation Using HiBiT



Iris K.A. Jones and Daniel N. Streblow







12. Use of a Phosphoproteomic Screen to Profile Early Changes during HCMV Infection of Human Monocytes



Liudmila S. Chesnokova and Andrew D. Yurochko







13. A Generally Applicable CRISPR/Cas9 Screening Technique to Identify Host Genes Required for Virus Infection as Applied to Human Cytomegalovirus (HCMV) Infection of Epithelial Cells



Xiaofei E and Timothy F. Kowalik







14. Quantitative Electron Microscopy to Study HCMV Morphogenesis



Clarissa Read, Paul Walther, and Jens von Einem







15. Detection of Cytomegalovirus Interleukin 10 (cmvIL-10) by Enzyme-Linked Immunosorbent Assay (ELISA)



Vivian P. Young, Margarette C. Mariano, Lionel Faure, and Juliet V. Spencer







16. Techniques for Characterizing Cytomegalovirus-Encoded miRNAs



Nicole L. Diggins, Lindsey B. Crawford, Hillary M. Struthers, Lauren M. Hook, Igor Landais, Rebecca L. Skalsky, and Meaghan H. Hancock







17. Development of a huBLT Mouse Model to Study HCMV Latency, Reactivation, and Immune Response



Lindsey B. Crawford and Patrizia Caposio







18. Rodent Models of Congenital Cytomegalovirus Infection



Berislav Lisnic, Jelena Tomac, Djurdjica Cekinovic, Stipan Jonjic, and Vanda Juranic Lisnic







19. Recent Approaches and Strategies in the Generation of Anti-Human Cytomegalovirus Vaccines



Suresh B. Boppana and William J. Britt

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