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The epithelial-to mesenchymal transition : methods and protocols

The epithelial-to mesenchymal transition : methods and protocols

Material type
단행본
Personal Author
Campbell, Kyra. Theveneau, Eric.
Title Statement
The epithelial-to mesenchymal transition : methods and protocols / edited by Kyra Campbell, Eric Theveneau.
Publication, Distribution, etc
New York :   Humana Press,   c2021.  
Physical Medium
xv, 429 p. : col. ill. ; 26 cm.
Series Statement
Methods in molecular biology,1064-3745 ; 2179
ISBN
9781071607787
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Mesenchymal stem cells --Laboratory manuals. Epithelial cells --Laboratory manuals.
000 00000nam u2200205 a 4500
001 000046092231
005 20210913135657
008 210913s2021 nyua b 001 0 eng d
020 ▼a 9781071607787
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 616.02774 ▼2 23
084 ▼a 616.02774 ▼2 DDCK
090 ▼a 616.02774 ▼b E642
245 0 4 ▼a The epithelial-to mesenchymal transition : ▼b methods and protocols / ▼c edited by Kyra Campbell, Eric Theveneau.
260 ▼a New York : ▼b Humana Press, ▼c c2021.
300 ▼a xv, 429 p. : ▼b col. ill. ; ▼c 26 cm.
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 2179
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
650 0 ▼a Mesenchymal stem cells ▼v Laboratory manuals.
650 0 ▼a Epithelial cells ▼v Laboratory manuals.
700 1 ▼a Campbell, Kyra.
700 1 ▼a Theveneau, Eric.
830 0 ▼a Methods in molecular biology ; ▼v 2179.
830 0 ▼a Springer protocols.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 616.02774 E642 Accession No. 521006077 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

Part I: Perspectives on the Past and Future of EMT Research



1. A Spectrum of Cell States during the Epithelial-To-Mesenchymal Transition



Erica J. Hutchins and Marianne E. Bronner







2. Perspective on Epithelial Mesenchymal Transitions in Embryos



David R. McClay







3. EMT, One of Many Morphological Transitions in Cellular Phase Space



Denise J. Montell







4. Are you Interested or Afraid of Working on EMT?



M. Angela Nieto







5. Partial EMT/MET: An Army of One



Sofiane Hamidi, Hiroki Nagai, and Guojun Sheng







6. EMT; An Update



Jean Paul Thiery







Part II: Review on Mesenchymal-to-Epithelial Transitions



7. Mesenchymal to Epithelial Transitions in Development and Cancer



John-Poul Ng-Blichfeldt and Katja Roeper







Part III: Methods to Study EMT in In Vivo Systems



8. Live Imaging of Epithelial-mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos



Lingkun Gu and Mo Weng







9. Zebrafish Neural Crest: Lessons and Tools to Study in vivo Cell Migration



Zain Alhashem, Macarena Alvarez-Garcillan Portillo, Ravinand Htun, Anton Gauert, Luis Briones Montecinos, Steffen Hartel, and Claudia Linker







10. Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo



Mary Cathleen McKinney and Paul M. Kulesa







11. Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors



Sofia Golenkina, Rosemary Manhire-Heath, and Michael J. Murray







12. Visualizing Mouse Embryo Gastrulation Epithelial-mesenchymal Transition through Single Cell Labeling Followed by ex vivo Whole Embryo Live Imaging



Wallis Nahaboo, Bechara Saykali, Navrita Mathiah, and Isabelle Migeotte







13. Fluorescence Recovery after Photobleaching to Study the Dynamics of Membrane Bound Proteins in vivo using the Drosophila Embryo



Joshua Greig and Natalia A. Bulgakova







14. Methods to Generate and Assay for Distinct Stages of Cancer Metastasis in adult Drosophila melanogaster



Jamie Adams, Andreu Casali, and Kyra Campbell







Part IV: In Vivo Imaging of MET



15. 4D Live-Imaging and Analysis of Chick Embryo Somites



Gi Fay Mok, James McColl, and Andrea Munsterberg







16. In vivo Analysis of the Mesenchymal-to-Epithelial Transition during Chick Secondary Neurulation



Elena Gonzalez-Gobartt, Guillaume Allio, Bertrand Benazeraf, and Elisa Marti







17. Multiscale in vivo Imaging of Collective Cell Migration in Drosophila Embryos



Gordana Scepanovic, Alexandru Florea, and Rodrigo Fernandez-Gonzalez







Part V: Ex Vivo/InVitro Methods to Study EMT/MEt



18. Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering



Minerva Bosch-Fortea and Fernando Martin-Belmonte







19. CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay



Sefora Conti, Takuya Kato, Danielle Park, Erik Sahai, Xavier Trepat, Anna Labernadie







20. Using Xenopus Neural Crest Explants to Study



Epithelial-mesenchymal Transition



Nadege Gouignard, Christian Rouviere, and Eric Theveneau







21. Xenopus Deep Cell Aggregates: a 3D Tissue Model for Mesenchymal-To-Epithelial Transition



Hye Young Kim and Lance A. Davidson







22. Molecular Tension Microscopy of E-cadherin during Epithelial-Mesenchymal Transition



Helena Canever, Pietro Salvatore Carollo, Romain Fleurisson, Philippe P. Girard, and Nicolas Borghi







Part VI: Methods to Study Epithelial- Mesenhymal Plasticity at the Single Cell, Subcellular, and Molecular Level



23. Methodologies for Following EMT in vivo at Single Cell Resolution



Abdull J. Massri, Geoffrey R. Schiebinger, Alejandro Berrio, Lingyu Wang, Gregory A. Wray, and David R. McClay







24. Isolation and Identification of EMT Subtypes



Robert J. Norgard and Ben Z. Stanger







25. Studying the Metabolism of Epithelial-Mesenchymal Plasticity using the Seahorse XFe96 Extracellular Flux Analyzer



Sugandha Bhatia, Erik W Thompson, and Jennifer H Gunter







26. Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells



Cecile Davaine, Eva Hadadi, William Taylor, Annelise Bennaceur-Griscelli, and Herve Acloque







27. Analysis of cellular EMT States using Molecular Biology and High Resolution FISH Labeling



Noemie Kempf, Fatima Moutahir, Isabelle Goiffon, Sylvain Cantaloube, Kerstin Bystricky, and Anne-Claire Lavigne







28. Mathematical Modeling of Plasticity and Heterogeneity in EMT



Shubham Tripathi, Jianhua Xing, Herbert Levine, and Mohit Kumar Jolly







29. Control of Cell Migration Using Optogenetics



Leo Valon and Simon de Beco

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