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The epithelial-to mesenchymal transition : methods and protocols

The epithelial-to mesenchymal transition : methods and protocols

Material type
Personal Author
Campbell, Kyra. Theveneau, Eric.
Title Statement
The epithelial-to mesenchymal transition : methods and protocols / edited by Kyra Campbell, Eric Theveneau.
Publication, Distribution, etc
New York :   Humana Press,   c2021.  
Physical Medium
xv, 429 p. : col. ill. ; 26 cm.
Series Statement
Methods in molecular biology,1064-3745 ; 2179
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Mesenchymal stem cells --Laboratory manuals. Epithelial cells --Laboratory manuals.
000 00000nam u2200205 a 4500
001 000046092231
005 20210913135657
008 210913s2021 nyua b 001 0 eng d
020 ▼a 9781071607787
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 616.02774 ▼2 23
084 ▼a 616.02774 ▼2 DDCK
090 ▼a 616.02774 ▼b E642
245 0 4 ▼a The epithelial-to mesenchymal transition : ▼b methods and protocols / ▼c edited by Kyra Campbell, Eric Theveneau.
260 ▼a New York : ▼b Humana Press, ▼c c2021.
300 ▼a xv, 429 p. : ▼b col. ill. ; ▼c 26 cm.
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 2179
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
650 0 ▼a Mesenchymal stem cells ▼v Laboratory manuals.
650 0 ▼a Epithelial cells ▼v Laboratory manuals.
700 1 ▼a Campbell, Kyra.
700 1 ▼a Theveneau, Eric.
830 0 ▼a Methods in molecular biology ; ▼v 2179.
830 0 ▼a Springer protocols.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 616.02774 E642 Accession No. 521006077 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

Part I: Perspectives on the Past and Future of EMT Research

1. A Spectrum of Cell States during the Epithelial-To-Mesenchymal Transition

Erica J. Hutchins and Marianne E. Bronner

2. Perspective on Epithelial Mesenchymal Transitions in Embryos

David R. McClay

3. EMT, One of Many Morphological Transitions in Cellular Phase Space

Denise J. Montell

4. Are you Interested or Afraid of Working on EMT?

M. Angela Nieto

5. Partial EMT/MET: An Army of One

Sofiane Hamidi, Hiroki Nagai, and Guojun Sheng

6. EMT; An Update

Jean Paul Thiery

Part II: Review on Mesenchymal-to-Epithelial Transitions

7. Mesenchymal to Epithelial Transitions in Development and Cancer

John-Poul Ng-Blichfeldt and Katja Roeper

Part III: Methods to Study EMT in In Vivo Systems

8. Live Imaging of Epithelial-mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos

Lingkun Gu and Mo Weng

9. Zebrafish Neural Crest: Lessons and Tools to Study in vivo Cell Migration

Zain Alhashem, Macarena Alvarez-Garcillan Portillo, Ravinand Htun, Anton Gauert, Luis Briones Montecinos, Steffen Hartel, and Claudia Linker

10. Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo

Mary Cathleen McKinney and Paul M. Kulesa

11. Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors

Sofia Golenkina, Rosemary Manhire-Heath, and Michael J. Murray

12. Visualizing Mouse Embryo Gastrulation Epithelial-mesenchymal Transition through Single Cell Labeling Followed by ex vivo Whole Embryo Live Imaging

Wallis Nahaboo, Bechara Saykali, Navrita Mathiah, and Isabelle Migeotte

13. Fluorescence Recovery after Photobleaching to Study the Dynamics of Membrane Bound Proteins in vivo using the Drosophila Embryo

Joshua Greig and Natalia A. Bulgakova

14. Methods to Generate and Assay for Distinct Stages of Cancer Metastasis in adult Drosophila melanogaster

Jamie Adams, Andreu Casali, and Kyra Campbell

Part IV: In Vivo Imaging of MET

15. 4D Live-Imaging and Analysis of Chick Embryo Somites

Gi Fay Mok, James McColl, and Andrea Munsterberg

16. In vivo Analysis of the Mesenchymal-to-Epithelial Transition during Chick Secondary Neurulation

Elena Gonzalez-Gobartt, Guillaume Allio, Bertrand Benazeraf, and Elisa Marti

17. Multiscale in vivo Imaging of Collective Cell Migration in Drosophila Embryos

Gordana Scepanovic, Alexandru Florea, and Rodrigo Fernandez-Gonzalez

Part V: Ex Vivo/InVitro Methods to Study EMT/MEt

18. Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering

Minerva Bosch-Fortea and Fernando Martin-Belmonte

19. CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay

Sefora Conti, Takuya Kato, Danielle Park, Erik Sahai, Xavier Trepat, Anna Labernadie

20. Using Xenopus Neural Crest Explants to Study

Epithelial-mesenchymal Transition

Nadege Gouignard, Christian Rouviere, and Eric Theveneau

21. Xenopus Deep Cell Aggregates: a 3D Tissue Model for Mesenchymal-To-Epithelial Transition

Hye Young Kim and Lance A. Davidson

22. Molecular Tension Microscopy of E-cadherin during Epithelial-Mesenchymal Transition

Helena Canever, Pietro Salvatore Carollo, Romain Fleurisson, Philippe P. Girard, and Nicolas Borghi

Part VI: Methods to Study Epithelial- Mesenhymal Plasticity at the Single Cell, Subcellular, and Molecular Level

23. Methodologies for Following EMT in vivo at Single Cell Resolution

Abdull J. Massri, Geoffrey R. Schiebinger, Alejandro Berrio, Lingyu Wang, Gregory A. Wray, and David R. McClay

24. Isolation and Identification of EMT Subtypes

Robert J. Norgard and Ben Z. Stanger

25. Studying the Metabolism of Epithelial-Mesenchymal Plasticity using the Seahorse XFe96 Extracellular Flux Analyzer

Sugandha Bhatia, Erik W Thompson, and Jennifer H Gunter

26. Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells

Cecile Davaine, Eva Hadadi, William Taylor, Annelise Bennaceur-Griscelli, and Herve Acloque

27. Analysis of cellular EMT States using Molecular Biology and High Resolution FISH Labeling

Noemie Kempf, Fatima Moutahir, Isabelle Goiffon, Sylvain Cantaloube, Kerstin Bystricky, and Anne-Claire Lavigne

28. Mathematical Modeling of Plasticity and Heterogeneity in EMT

Shubham Tripathi, Jianhua Xing, Herbert Levine, and Mohit Kumar Jolly

29. Control of Cell Migration Using Optogenetics

Leo Valon and Simon de Beco

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