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Melanoma : methods and protocols

Melanoma : methods and protocols

자료유형
단행본
개인저자
Hargadon, Kristian M.
서명 / 저자사항
Melanoma : methods and protocols / edited by Kristian M. Hargadon.
발행사항
New York :   Humana Press,   c2021.  
형태사항
xx, 674 p. : ill. (some col.) ; 27 cm.
총서사항
Methods in molecular biology,1064-3745 ; 2265
ISBN
9781071612040
서지주기
Includes bibliographical references and index.
일반주제명
Melanoma.
000 00000nam u2200205 a 4500
001 000046085603
005 20210709172032
008 210709s2021 nyua b 001 0 eng d
020 ▼a 9781071612040
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 616.99477 ▼2 23
084 ▼a 616.99477 ▼2 DDCK
090 ▼a 616.99477 ▼b M517
245 0 0 ▼a Melanoma : ▼b methods and protocols / ▼c edited by Kristian M. Hargadon.
260 ▼a New York : ▼b Humana Press, ▼c c2021.
300 ▼a xx, 674 p. : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 2265
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
650 0 ▼a Melanoma.
700 1 ▼a Hargadon, Kristian M.
830 0 ▼a Methods in molecular biology ; ▼v 2265.
830 0 ▼a Springer protocols.
945 ▼a KLPA

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 616.99477 M517 등록번호 521005983 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

목차

1. Model Systems for the Study of Malignant Melanoma



Randal K. Gregg







Part I: 2D Cell Culture-Based Approaches for Studying Melanoma Biology







2. Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing



Kristian M. Hargadon, David Z. Bushhouse, Coleman E. Johnson, and Corey J. Willams







3. A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix

Ewa Mazurkiewicz, Ewa Mrowczynska, Aleksandra Simiczyjew, Dorota Nowak, and Antonina J. Mazur







4. Wound Healing Assay for Melanoma Cell Migration



Juliano T. Freitas, Ivan Jozic, and Barbara Bedogni







5. A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells



Jelena Grahovac, Marijana Pavlovic, and Marija Vidosavljevic







6. Analyzing Melanoma Cell Oxygen Consumption and Extracellular Acidification Rates Using Seahorse Technology



Ashley V. Menk and Greg M. Delgoffe





7. Analysis of Melanoma Cell Glutamine Metabolism by Stable Isotope Tracing and Gas Chromatography-Mass Spectrometry



David A. Scott







8. Determination of Cytotoxic Activities Against Melanoma Cells Using Flow Cytometry



Guilan Shi and Richard Heller





9. In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium



Tao Wang and Russel E. Kaufman







10. A Flow Cytometric Assay for Investigating Melanoma Cell Adhesion to Lymphatic Endothelial Cells



Kristian M. Hargadon and Coleman E. Johnson







Part II: 3D Cell Culture Systems for Studying Melanoma







11. An Approach to Study Melanoma Invasion and Crosstalk with Lymphatic Endothelial Cell Spheroids in 3D Using Immunofluorescence



Sanni Alve, Silvia Gramolelli, and Paivi M. Ojala







12. Evaluating Melanoma Viability and Proliferation in 3D Microenvironments



Vasanth Siruvallur Murali, Murat Can Cobanoglu, and Erik S. Welf







13. Preparation, Drug Treatment, and Immunohistological Analysis of Tri-Culture Spheroid 3D Melanoma-Like Models



Maximilian E.A. Schafer, Julia Klicks, Mathias Hafner, and Rudiger Rudolf







14. Enrichment of Melanoma Cancer Stem Cells via Sphere Assays



Nabanita Mukherjee, Karoline A. Lambert, David A. Norris, and Yiqun G. Shellman







Part III: Techniques for Isolating and Studying Circulating Melanoma Cells







15. Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry



Robert H. Edgar, Justin Cook, Madeline Douglas, Anie-Pier Samson, and John A. Viator







16. Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells



Aaron B. Beasley, Emmanuel Acheampong, Weitao Lin, and Elin S. Gray







17. PD-L1 Detection on Circulating Melanoma Cells



Joseph W. Po, Yafeng Ma, Bavanthi Balakrishnar, Daniel Brungs, Farhad Azimi, Adam Cooper, Erin Saricilar, Vinay Murthy, Paul de Souza, and Therese M. Becker







18. Transcript-Based Detection of Circulating Melanoma Cells



Michael Morici, Weitao Lin, and Elin S. Gray







19. Isolation and Quantification of Plasma Circulating Tumor DNA from Melanoma Patients



Gabriela Marsavela, Anna Reid, Elin S. Gray, and Leslie Calapre







20. Simultaneous BRAFV600E Protein and DNA Aberration Detection in Circulating Melanoma Cells Using an Integrated Multi-Molecular Sensor



Alain Wuethrich, Shuvashis Dey, Kevin M. Koo, Abu A.I. Sina, and Matt Trau







21. Single Cell Analysis of BRAFV600E and NRASQ61R Mutation Status in Melanoma Cell Lines as Method Generation for Circulating Melanoma Cells



Joseph W. Po, Yafeng Ma, Alison W.S. Luk, David Lynch, Bavanthi Balakrishnar, Daniel Brungs, Farhad Azimi, Adam Cooper, Erin Saricilar, Vinay Murthy, Paul de Souza, and Therese M. Becker







Part IV: Methods to Study Melanoma-Derived and Melanoma-Associated Exosomes







22. A Rapid Exosome Isolation Using Size Exclusion Chromatography (REIUS) Method for Exosome Isolation from Melanoma Cell Lines



Shin La Shu, Cheryl L. Allen, Shawna Benjamin-Davalos, Marina Koroleva, Don MacFarland, Hans Minderman, and Marc S. Ernstoff





23. Immunoaffinity-Based Isolation of Melanoma Cell-Derived and T Cell-Derived Exosomes from Plasma of Melanoma Patients



Sujan Kumar Mondal and Theresa L. Whiteside







24. An Immunocapture-Based Assay for Detecting Multiple Antigens in Melanoma-Derived Extracellular Vesicles



Carmen Campos-Silva, Yaiza Caceres-Martell, Sheila Lopez-Cobo, Maria Josefa Rodriguez, Ricardo Jara, Maria Yanez-Mo, and Mar Vales-Gomez







25. Post-Lymphadenectomy Analysis of Exosomes from Lymphatic Exudate/Exudative Seroma of Melanoma Patients



Susana Garcia-Silva, Pilar Ximenez-Embun, Javier Munoz, and Hector Peinado







Part V: Assessing Melanoma Metastasis in Pre-Clinical and Clinical Settings







26. Fate Mapping of Cancer Cells in Metastatic Lymph Nodes Using Photoconvertible Proteins



Ethel R. Pereira, Dmitriy Kedrin, and Timothy P. Padera







27. Detection of Melanoma Cells in Lymphatic Drainage (LD) after Lymph Nodes Dissection via Nested RT-PCR Analysis of Molecular Melanocytic Markers



Aleksandra Gos, Piotr Rutkowski, and Janusz A. Siedlecki





28. A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue



Fabrizio Mattei, Sara Andreone, and Giovanna Schiavoni







29. PET Imaging of Melanoma Using Melanin-Targeted Probe



Xiaowei Ma and Zhen Cheng







30. Imaging and Isolation of Extravasation-Participating Endothelial and Melanoma Cells During Angiopellosis



Tyler A. Allen and Ke Cheng







Part VI: Strategies for Diagnostic and Prognostic Biomarker Identification for Melanoma and Computational/Bioinformatic Approaches to OMICS Analysis of Melanoma







31. Salivary Proteomic Analysis of Canine Oral Melanoma by MALDI-TOF Mass Spectrometry and LC-Mass Spectrometry/Mass Spectrometry



Sekkarin Ploypetch, Sittiruk Roytrakul, and Gunnaporn Suriyaphol







32. Detection of Uveal Melanoma by Multiplex Immunoassays of Serum Biomarkers



Jin Song, Zhen Zhang, and Daniel W. Chan







33. Unbiased Microbiome and Metabolomic Profiling of Fecal Samples from Patients with Melanoma



Ashley Bui, Yongbin Choi, Arthur E. Frankel, and Andrew Y. Koh







34. Assessment of Cell-Free microRNA by NGS Whole Transcriptome Analysis in Cutaneous Melanoma Patients'' Blood



Kevin D. Tran, Rebecca Gross, Negin Rahimzadeh, Shanthy Chenathukattil, Dave S.B. Hoon, and Matias A. Bustos







35. High-Throughput Identification of miRNA-Target Interactions in Melanoma Using miR-CATCHv2.0



Andrea Marranci, Romina D''Aurizio, Milena Rizzo, Catherine M. Greene, and Laura Poliseno







Part VII: Characterization of Melanoma-Associated Tumor-Infiltrating Lymphocytes and Tertiary Lymphoid Structures







36. Immunotyping and Quantification of Melanoma Tumor-Infiltrating Lymphocytes



Max O. Meneveau, Zeyad T. Sahli, Kevin T. Lynch, Ileana S. Mauldin, and Craig L. Slingluff, Jr.







37. Single Cell Gene Expression, Clonality, and Feature Barcoding of Melanoma Tumor Infiltrating Lymphocytes



Angela Pizzolla, Simon Keam, Criselle D''Souza, Timothy Semple, and Paul J. Neeson







38. Using Mass Cytometry to Analyze the Tumor-Infiltrating Lymphocytes in Human Melanoma



Daniela Tantalo, Thu Nguyen, Han Xian Aw Yeang, Joe Zhu, Sean Macdonald, Minyu Wang, Harini de Silva, Criselle D''Souza, Angela Pizzolla, and Paul J. Neeson







39. Multiplex Immunohistochemistry Analysis of Melanoma Tumor Infiltrating Lymphocytes



Thu Nguyen, Nikolce Kocovski, Sean Macdonald, Han Xian Aw Yeang, Minyu Wang, and Paul J. Neeson







40. Multiplex Immunofluorescence Histology for Immune Cell Infiltrates in Melanoma-Associated Tertiary Lymphoid Structures



Ileana S. Mauldin, Adela Mahmutovic, Samuel J. Young, and Craig L. Slingluff, Jr.







Part VIII: Development and Evaluation of Therapeutic Strategies for Melanoma Treatment







41. The Use of Nanoparticles in the Delivery of Nucleic Acids for Melanoma Treatment



Mohammad A. Obeid, Alaa A.A. Aljabali, Meriem Rezigue, Haneen Amawi, Hanin Alyamani, Shatha N. Abdeljaber, and Valerie A. Ferro







42. siRNA Delivery to Melanoma Cells with Cationic Niosomes



Mohammad A. Obeid, Hanin Alyamani, Haneen Amawi, Alaa A.A. Aljabali, Meriem Rezigue, Shatha N. Abdeljaber, and Valerie A. Ferro







43. Controlled Delivery of Plasmid DNA to Melanoma Tumors by Gene Electrotransfer



Richard Heller and Guilan Shi







44. Generation of Murine Chimeric Antigen Receptor T Cells for Adoptive T Cell Therapy for Melanoma



Amorette Barber







45. Generation of Phosphopeptide-Specific T Cell Lines as Tools for Melanoma Immunotherapy



Rebecca C. Obeng and Angela L. Ambakhutwala

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