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Chondrocytes : methods and protocols

Chondrocytes : methods and protocols

Material type
Personal Author
Haqqi, Tariq M., editor. Lefebvre, Véronique, editor.
Title Statement
Chondrocytes : methods and protocols / edited by Tariq M. Haqqi, Véronique Lefebvre.
Publication, Distribution, etc
New York :   Humana Press,   c2021.  
Physical Medium
xiii, 229 p. : ill. (some col.) ; 26 cm.
Series Statement
Methods in molecular biology ;2245
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Cartilage cells --Laboratory manuals. Cartilage cells.
000 00000nam u2200205 a 4500
001 000046077726
005 20210419114121
008 210419s2021 nyua b 001 0 eng d
020 ▼a 9781071611180
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 612.75176 ▼2 23
084 ▼a 612.75176 ▼2 DDCK
090 ▼a 612.75176 ▼b C548
245 0 0 ▼a Chondrocytes : ▼b methods and protocols / ▼c edited by Tariq M. Haqqi, Véronique Lefebvre.
260 ▼a New York : ▼b Humana Press, ▼c c2021.
300 ▼a xiii, 229 p. : ▼b ill. (some col.) ; ▼c 26 cm.
490 1 ▼a Methods in molecular biology ; ▼v 2245
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
650 0 ▼a Cartilage cells ▼v Laboratory manuals.
650 0 ▼a Cartilage cells.
700 1 ▼a Haqqi, Tariq M., ▼e editor.
700 1 ▼a Lefebvre, Véronique, ▼e editor.
830 0 ▼a Methods in molecular biology ; ▼v 2245.
830 0 ▼a Springer protocols.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 612.75176 C548 Accession No. 121257072 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

1. Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D

Rodrigo A. Somoza and Jean F. Welter

2. Protocol for the Isolation of Intact Chondrons from Healthy and Osteoarthritic Human Articular Cartilage

Ilona Uzieliene, Jaroslav Denkovskij, Eiva Bernotiene, Ursule Kalvaityte, Raminta Vaiciuleviciute, Yolande F.M. Ramos, and Ali Mobasheri

3. Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse

Ioannis Kanakis, Mohammad Alhashmi, Ke Liu, Craig Keenan, Lorenzo Ramos Mucci, Blandine Poulet, and George Bou-Gharios

4. Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures

Abdul Haseeb and Veronique Lefebvre

5. Fetal Growth Plate Cartilage: Histological and Immunohistochemical Techniques

Zachary Tata, Christophe Merceron, and Ernestina Schipani

6. Preparation of Adult Mouse Skeletal Tissue Sections for RNA In Situ Hybridization

Charles de Charleroy, Abdul Haseeb, and Veronique Lefebvre

7. MicroRNA In Situ Hybridization in Paraffin-Embedded Human Articular Cartilage and Mouse Knee Joints

Helal Endisha and Mohit Kapoor

8. Laser Capture Microdissection of Mouse Growth Plate Cartilage

Bijal Kikani and Julian C. Lui

9. RNA Isolation from Articular Cartilage Tissue

Hongjun Zheng and Audrey McAlinden

10. Small Nucleolar RNA Expression Profiling in Cartilage

Mandy J. Peffers, Andy Cremers, and Tim J.M. Welting

11. MicroRNA Expression Profiling, Target Identification, and Validation in Chondrocytes

Tomoki Chiba, Ryota Kurimoto, Takahide Matsushima, Yoshiaki Ito, Ryo Nakamichi, Martin Lotz, and Hiroshi Asahara

12. ChIP-Seq Assays from Mammalian Cartilage and Chondrocytes

Akira Yamakawa, Hironori Hojo, and Shinsuke Ohba

13. MicroRNAs and Regulation of Autophagy in Chondrocytes

Yolande F.M. Ramos and Ali Mobasheri

14. Mitochondrial Biogenesis, Activity, and DNA Isolation in Chondrocytes

Yun Wang, Liang-Yu Chen, and Ru Liu-Bryan

15. Assessing Chondrocyte Status by Immunofluorescence-Mediated Localization of Parkin Relative to Mitochondria

Mohammad Y. Ansari and Tariq M. Haqqi

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