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Chondrocytes : methods and protocols

Chondrocytes : methods and protocols

Material type
단행본
Personal Author
Haqqi, Tariq M., editor. Lefebvre, Véronique, editor.
Title Statement
Chondrocytes : methods and protocols / edited by Tariq M. Haqqi, Véronique Lefebvre.
Publication, Distribution, etc
New York :   Humana Press,   c2021.  
Physical Medium
xiii, 229 p. : ill. (some col.) ; 26 cm.
Series Statement
Methods in molecular biology ;2245
ISBN
9781071611180
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Cartilage cells --Laboratory manuals. Cartilage cells.
000 00000nam u2200205 a 4500
001 000046077726
005 20210419114121
008 210419s2021 nyua b 001 0 eng d
020 ▼a 9781071611180
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 612.75176 ▼2 23
084 ▼a 612.75176 ▼2 DDCK
090 ▼a 612.75176 ▼b C548
245 0 0 ▼a Chondrocytes : ▼b methods and protocols / ▼c edited by Tariq M. Haqqi, Véronique Lefebvre.
260 ▼a New York : ▼b Humana Press, ▼c c2021.
300 ▼a xiii, 229 p. : ▼b ill. (some col.) ; ▼c 26 cm.
490 1 ▼a Methods in molecular biology ; ▼v 2245
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
650 0 ▼a Cartilage cells ▼v Laboratory manuals.
650 0 ▼a Cartilage cells.
700 1 ▼a Haqqi, Tariq M., ▼e editor.
700 1 ▼a Lefebvre, Véronique, ▼e editor.
830 0 ▼a Methods in molecular biology ; ▼v 2245.
830 0 ▼a Springer protocols.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 612.75176 C548 Accession No. 121257072 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

1. Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D



Rodrigo A. Somoza and Jean F. Welter







2. Protocol for the Isolation of Intact Chondrons from Healthy and Osteoarthritic Human Articular Cartilage



Ilona Uzieliene, Jaroslav Denkovskij, Eiva Bernotiene, Ursule Kalvaityte, Raminta Vaiciuleviciute, Yolande F.M. Ramos, and Ali Mobasheri







3. Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse



Ioannis Kanakis, Mohammad Alhashmi, Ke Liu, Craig Keenan, Lorenzo Ramos Mucci, Blandine Poulet, and George Bou-Gharios







4. Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures



Abdul Haseeb and Veronique Lefebvre







5. Fetal Growth Plate Cartilage: Histological and Immunohistochemical Techniques



Zachary Tata, Christophe Merceron, and Ernestina Schipani







6. Preparation of Adult Mouse Skeletal Tissue Sections for RNA In Situ Hybridization



Charles de Charleroy, Abdul Haseeb, and Veronique Lefebvre







7. MicroRNA In Situ Hybridization in Paraffin-Embedded Human Articular Cartilage and Mouse Knee Joints



Helal Endisha and Mohit Kapoor







8. Laser Capture Microdissection of Mouse Growth Plate Cartilage



Bijal Kikani and Julian C. Lui







9. RNA Isolation from Articular Cartilage Tissue



Hongjun Zheng and Audrey McAlinden







10. Small Nucleolar RNA Expression Profiling in Cartilage



Mandy J. Peffers, Andy Cremers, and Tim J.M. Welting







11. MicroRNA Expression Profiling, Target Identification, and Validation in Chondrocytes



Tomoki Chiba, Ryota Kurimoto, Takahide Matsushima, Yoshiaki Ito, Ryo Nakamichi, Martin Lotz, and Hiroshi Asahara







12. ChIP-Seq Assays from Mammalian Cartilage and Chondrocytes



Akira Yamakawa, Hironori Hojo, and Shinsuke Ohba







13. MicroRNAs and Regulation of Autophagy in Chondrocytes



Yolande F.M. Ramos and Ali Mobasheri







14. Mitochondrial Biogenesis, Activity, and DNA Isolation in Chondrocytes



Yun Wang, Liang-Yu Chen, and Ru Liu-Bryan







15. Assessing Chondrocyte Status by Immunofluorescence-Mediated Localization of Parkin Relative to Mitochondria



Mohammad Y. Ansari and Tariq M. Haqqi

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