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Ewing sarcoma : methods and protocols

Ewing sarcoma : methods and protocols

Material type
단행본
Personal Author
Cidre-Aranaz, Florencia, editor. Grünewald, Thomas G. P., editor.
Title Statement
Ewing sarcoma : methods and protocols / edited by Florencia Cidre-Aranaz, Thomas G. P. Grünewald.
Publication, Distribution, etc
New York :   Humana Press,   c2021.  
Physical Medium
xi, 337 p. : ill. (some col.) ; 26 cm.
Series Statement
Methods in molecular biology ;2226
ISBN
9781071610190
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Ewing's sarcoma.
000 00000nam u2200205 a 4500
001 000046077724
005 20210419111657
008 210419s2021 nyua b 001 0 eng d
020 ▼a 9781071610190
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 616.994716 ▼2 23
084 ▼a 616.994716 ▼2 DDCK
090 ▼a 616.994716 ▼b E95
245 0 0 ▼a Ewing sarcoma : ▼b methods and protocols / ▼c edited by Florencia Cidre-Aranaz, Thomas G. P. Grünewald.
260 ▼a New York : ▼b Humana Press, ▼c c2021.
300 ▼a xi, 337 p. : ▼b ill. (some col.) ; ▼c 26 cm.
490 1 ▼a Methods in molecular biology ; ▼v 2226
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
650 0 ▼a Ewing's sarcoma.
700 1 ▼a Cidre-Aranaz, Florencia, ▼e editor.
700 1 ▼a Grünewald, Thomas G. P., ▼e editor.
830 0 ▼a Methods in molecular biology ; ▼v 2226.
830 0 ▼a Springer protocols.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 616.994716 E95 Accession No. 121257070 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

Part I: Introduction



1. Germline Variation and Somatic Alterations in Ewing Sarcoma



Mitchell J. Machiela and Thomas G. P. Grunewald







2. Western Blot Analysis in Ewing Sarcoma



Aruna Marchetto and Laura Romero-Perez







Part II: Molecular Biology Techniques Applied To Ewing Sarcoma



3. Tissue Preservation and FFPE Samples: Optimized Nucleic Acids Isolation in Ewing Sarcoma



Laura Romero-Perez and Thomas G. P. Grunewald







4. Liquid Biopsies in Ewing Sarcoma



Manuela Krumbholz and Markus Metzler







5. (Immuno-)histological Analysis of Ewing Sarcoma



David Marcilla, Isidro Machado, Thomas G. P. Grunewald, Antonio Llombart-Bosch, and Enrique de Alava







6. Molecular Approaches to Diagnosis In Ewing Sarcoma: Fluorescence in situ hybridization (FISH)



Marcel Trautmann and Wolfgang Hartmann







7. Molecular Approaches to Diagnosis in Ewing Sarcoma: RT-PCR



Carlos Rodriguez-Martin and Javier Alonso







8. Molecular Approaches to Diagnosis in Ewing Sarcoma: Targeted RNA Sequencing



Carmen Salguero-Aranda and Juan Diaz-Martin







Part III: Diagnostics in Ewing Sarcoma



9. Ewing-specific (re)expression Models



Maximilian M. L. Knott and Florencia Cidre-Aranaz







10. Analysis of Regulatory Sequences by Dual-luciferase Reporter Assays in Ewing Sarcoma



Tilman L. B. Hoelting and Maximilian M. L. Knott







Part IV: Cellular biology and cell culture techniques



11. Proliferation Assessment by Trypan Blue Exclusion in Ewing Sarcoma



Cornelius Funk and Julian Musa







12. Drug Screening by Resazurin Colorimetry in Ewing Sarcoma



Julian Musa and Florencia Cidre-Aranaz







13. Analysis of Migration and Invasion in Ewing Sarcoma



Florencia Cidre-Aranaz







Part V: Animal models in Ewing sarcoma



14. Genetically Engineered Mouse Model in Ewing Sarcoma



Miwa Tanaka and Takuro Nakamura







15. Tumor Growth Analysis of Ewing Sarcoma Cell Lines Using Subcutaneous Xenografts in Mice



Florencia Cidre-Aranaz and Shunya Ohmura







16. Metastasis Assessment in Ewing Sarcoma Using Orthotopic Xenografts



Roser Lopez-Alemany and Oscar M. Tirado







17. Orthotopic Implants in Mice



Elizabeth Stewart







18. Ewing Sarcoma PDX Models



D. Surdez, L. Landuzzi, K. Scotlandi, and M.C. Manara







Part VI: Bioinformatic approaches to Ewing sarcoma 19. Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma Susana Pascoal, Sarah Grissenberger, Eva Scheuringer, Rita Fior, Miguel Godinho Ferreira, and Martin Distel







20. Main Repositories and Databases used in Ewing Sarcoma Research



Maria Debiec-Rychter







21. Chromatin Immunoprecipitation Followed by Next-Generation Sequencing (ChIP-seq) Analysis in Ewing Sarcoma



Gwenneg Kerdivel and Valentina Boeva







22. Epigenetic Analysis in Ewing Sarcoma



Jeremy M. Simon and Nicholas C. Gomez







23. Systems Biology Analysis for Ewing Sarcoma



Marianyela Petrizzelli, Jane Merlevede, and Andrei Zinovyev

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