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Blocking transgelin(TAGLN) stimulates PLCγ1-Cofilin induced cytoskeletal remodeling and enhanced migration of MDA-MB-231

Blocking transgelin(TAGLN) stimulates PLCγ1-Cofilin induced cytoskeletal remodeling and enhanced migration of MDA-MB-231

자료유형
학위논문
개인저자
Raymundo, Bernardo R.
서명 / 저자사항
Blocking transgelin(TAGLN) stimulates PLCγ1-Cofilin induced cytoskeletal remodeling and enhanced migration of MDA-MB-231 / Bernardo R. Raymundo
발행사항
Seoul :   Greduate School, Korea University,   2021  
형태사항
iv, 53장 : 삽화, 도표 ; 26 cm
기타형태 저록
Blocking Transgelin(TAGLN) stimulates PLCγ1-Cofilin induced cytoskeletal remodeling and enhanced migration of MDA-MB-231   (DCOLL211009)000000235570  
학위논문주기
학위논문(박사)-- 고려대학교 대학원: 생명공학과, 2021. 2
학과코드
0510   6YD48   529  
일반주기
지도교수: 김찬화  
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참고문헌: 장 44-51
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PDF 파일로도 이용가능;   Requires PDF file reader(application/pdf)  
비통제주제어
Cofilin , Plasticity , Actin cytoskeleton,,
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100 1 ▼a Raymundo, Bernardo R.
245 1 0 ▼a Blocking transgelin(TAGLN) stimulates PLCγ1-Cofilin induced cytoskeletal remodeling and enhanced migration of MDA-MB-231 / ▼d Bernardo R. Raymundo
246 3 ▼a Blocking transgelin stimulates PLCγ1-Cofilin induced cytoskeletal remodeling and enhanced migration of MDA-MB-231
246 3 ▼a Blocking TAGLN stimulates PLCγ1-Cofilin induced cytoskeletal remodeling and enhanced migration of MDA-MB-231
260 ▼a Seoul : ▼b Greduate School, Korea University, ▼c 2021
300 ▼a iv, 53장 : ▼b 삽화, 도표 ; ▼c 26 cm
500 ▼a 지도교수: 김찬화
502 1 ▼a 학위논문(박사)-- ▼b 고려대학교 대학원: ▼c 생명공학과, ▼d 2021. 2
504 ▼a 참고문헌: 장 44-51
530 ▼a PDF 파일로도 이용가능; ▼c Requires PDF file reader(application/pdf)
653 ▼a Cofilin ▼a Plasticity ▼a Actin cytoskeleton
776 0 ▼t Blocking Transgelin(TAGLN) stimulates PLCγ1-Cofilin induced cytoskeletal remodeling and enhanced migration of MDA-MB-231 ▼w (DCOLL211009)000000235570
900 1 0 ▼a 김찬화, ▼g 金鑽和, ▼e 지도교수
900 0 0 ▼a 裵聞度
945 ▼a KLPA

전자정보

No. 원문명 서비스
1
Blocking transgelin(TAGLN) stimulates PLCγ1-Cofilin induced cytoskeletal remodeling and enhanced migration of MDA-MB-231
PDF 초록 목차

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/학위논문서고/ 청구기호 0510 6YD48 529 등록번호 123065664 도서상태 대출가능 반납예정일 예약 서비스 B M
No. 2 소장처 과학도서관/학위논문서고/ 청구기호 0510 6YD48 529 등록번호 123065665 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

초록

Abstract
The role of transgelin (TAGLN) in cancer has been discussed; however, the mechanisms underlying its regulation and correlation with MDA-MB-231 cell plasticity and migratory patterns remain unclear. I generated stable TAGLN-knockdown MDA-MB-231 cells and treated them with phorbol 12-myristate 13-acetate (PMA) or transforming growth factor (TGF)-β. Chemotaxis, morphology, and invasion were assayed using three-dimensional matrices to evaluate cytoskeletal remodeling and migratory changes. Wound healing assays were conducted using cell inserts. TAGLN knockdown cells exhibited altered morphology due to cytoskeletal remodeling, yet only untreated and TGFβ1-treated cells exhibited enhanced migration. Untreated and TGFβ1-treated TAGLN knockdown cells showed increased N-WASP, ROCK1, and ROCK2 protein levels, which induce cytoskeletal remodeling. Evaluating phospholipase Cγ1 (PLCγ1)-cofilin signaling-related proteins revealed that only TGFβ1-treated TAGLN knockdown cells were influenced by PLCγ1-cofilin signaling. Taken together, TAGLN knockdown is necessary for the TGFβ1-mediated activation of PLCγ1-cofilin pathway-driven amoeboid morphology and enhanced migratory properties in MDA-MB-231 cells.

목차

Abstract	1
I.	 Introduction	2
II.	Literature Review	5
2.1	Cellular plasticity in cancer	5
2.2	Actin binding proteins (ABP)	5
2.3	Transgelin ( TAGLN)	6
2.4	PLCγ1-cofilin signaling in cancer	6
3.1 Cell culture and transfection	8
3.2 Western blot analysis	9
3.3 Matrigel morphology assay	10
3.4 Chemotaxis assay	10
3.5 Cell motility assay (wound healing)	11
3.6 Transwell invasion assay	11
3.7 Adhesion assay	12
3.8 Statistical analysis	13
IV. Results	14
4.1 TAGLN knockdown induces an amoeboid-like morphology in MDA-MB231 cells	14
4.2 Initial TAGLN depletion leads to increased levels of proteins that induce amoeboid-like characteristics	19
4.3 PMA may reduce increased chemotactic responses to EGF, wound healing, and invasion of MDA-MB-231 cells after TAGLN depletion	22
4.4 TAGLN-positive cells exhibit the highest fibronectin adhesion	28
4.5 TAGLN-TGFβ axis-driven amoeboid and migratory properties of MDA-MB-231 cells are coupled to PLCγ1-cofilin signaling	28
V. Discussion	36
VI. Conclusion	42
VII. References	44
Abstract in Korean	52