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Cerebral cavernous malformations (CCM) : methods and protocols

Cerebral cavernous malformations (CCM) : methods and protocols

Material type
단행본
Personal Author
Trabalzini, Lorenza. Finetti, Federica. Retta, Saverio Francesco.
Title Statement
Cerebral cavernous malformations (CCM) : methods and protocols / edited by Lorenza Trabalzini, Federica Finetti, Saverio Francesco Retta.
Publication, Distribution, etc
New York :   Humana Press :   Springer,   c2020.  
Physical Medium
xv, 496 p. : ill. (chiefly col.) ; 27 cm.
Series Statement
Springer protocols
ISBN
9781071606391
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Brain --Abnormalities --Laboratory manuals.
000 00000nam u2200205 a 4500
001 000046044745
005 20200904114115
008 200901s2020 nyua b 001 0 eng d
020 ▼a 9781071606391
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 616.8/1 ▼2 23
084 ▼a 616.81 ▼2 DDCK
090 ▼a 616.81 ▼b C4145
245 0 0 ▼a Cerebral cavernous malformations (CCM) : ▼b methods and protocols / ▼c edited by Lorenza Trabalzini, Federica Finetti, Saverio Francesco Retta.
260 ▼a New York : ▼b Humana Press : ▼b Springer, ▼c c2020.
300 ▼a xv, 496 p. : ▼b ill. (chiefly col.) ; ▼c 27 cm.
490 1 ▼a Springer protocols
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 2152
504 ▼a Includes bibliographical references and index.
650 0 ▼a Brain ▼x Abnormalities ▼v Laboratory manuals.
700 1 ▼a Trabalzini, Lorenza.
700 1 ▼a Finetti, Federica.
700 1 ▼a Retta, Saverio Francesco.
830 0 ▼a Springer protocols.
830 0 ▼a Methods in molecular biology ; ▼v 2152.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 616.81 C4145 Accession No. 121254193 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

Part I: Overview of Cerebral Cavernous Malformations



1. From Genes and Mechanisms to Molecular-targeted Therapies: the Long Climb to the Cure of Cerebral Cavernous Malformation (CCM) Disease



Saverio Francesco Retta, Federica Finetti, Andrea Perrelli, and Lorenza Trabalzini







2. Incidence, Prevalence, and Clinical Presentation of Cerebral Cavernous Malformations



Kelly D. Flemming







3. Natural History, Clinical, and Surgical Management of Cavernous Malformations



Giovanni G. Vercelli, Fabio Cofano, Filippo Veneziani Santonio, Francesca Vincitorio, Francesco Zenga, and Diego Garbossa







Part II Diagnosis and Treatment of Cerebral Cavernous Malformations



4. Molecular Genetics Screening of CCM patients, An Overview



Elisabeth Tournier-Lasserve







5. Next Generation Sequecing (NGS) Strategies for Genetic Testing and Diagnosis of Cerebral Cavernous Malformation (CCM) Disease



Valerio Benedetti, Elisa Pellegrino, Alfredo Brusco, Roberto Piva, and Saverio Francesco Retta







6. Genome-wide Genotyping of Cerebral Cavernous Malformation Type 1 Individuals to Identify Genetic Modifiers of Disease Severity



Helene Choquet and Helen Kim







7. Clinical Imaging of Cerebral Cavernous Malformations: Computed Tomography and Magnetic Resonance Imaging



Marc Mabray and Blaine Hart







8. Neuroradiology: Differential Diagnosis, Follow-Up, and Reporting



Alessandra Splendiani, Federico Bruno, and Alfonso Cerase







9. Surgical Management of Brain Cavernous Malformations



Marco M. Fontanella, Luca Zanin, Alessandro Fiorindi, Giannantonio Spena, Federico Nicolosi, Francesco Belotti, Pierpaolo Panciani, Claudio Cornali, and Francesco Doglietto







Part III: Animal and Cellular Models of Cebral Cavernous Malformations



10. Generation of CCM Phenotype by a Human Microvascular Endothelial Model



Simona Delle Monache and Saverio Francesco Retta







11. Isolation and Purification of Mouse Brain Endothelial Cells to Study Cerebral Cavernous Malformation Disease



Preston Hale, Shady Ibrahim Soliman, Hao Sun, and Miguel Alejandro Lopez-Ramirez







12. Production of KRIT1-knockout and KRIT1-knockin Mouse Embryonic Fibroblasts as Cellular Models of CCM Disease



Luca Goitre, Claudia Fornelli, Alessia Zotta, Andrea Perrelli, and Saverio Francesco Retta







13. CRISPR/Cas9-mediated Generation of Human Endothelial Cell Knockout Models of CCM Disease



Konrad Schwefel, Stefanie Spiegler, Christiane D. Much, Ute Felbor, and Matthias Rath







14. Dissection of the Role of CCM Genes in Tubulogenesis using the Drosophila



Tracheal System as a Model



Alondra B. Schweizer Burguete and Amin S. Ghabrial







15. Generation and Analysis of CCM Phenotypes in C. elegans



Evelyn Popiel and William Brent Derry







16. Generation of Transgenic Lines of Zebrafish Expressing Fluorescently-tagged CCM Proteins to Study Their Function and Subcellular Localization within the Vasculature



Stefan Donat, and Salim Abdelilah-Seyfried







17. Vertebrate Models to Investigate CCM Pathogenesis: The Zebrafish and Mouse Model



Johnathan Abou-Fadel and Jun Zhang







18. Generation of Cerebral Cavernous Malformation in Neonatal Mouse Models using Inducible Cre-LoxP Strategy



Jaesung P. Choi and Xiangjian Zheng







19. Isolation of Cerebral Endothelial Cells from CCM1/KRIT1 Null Mouse Brain



Nicholas Nobiletti and Angela J. Glading







Part IV: Cellular and Molecular Mechanisms of Cerebral Cavernous Malformations



20. Identification of the KRIT1 Protein by LexA-based Yeast Two-Hybrid System



Ilya G. Serebriiskii, Mohamed Elmekawy, and Erica A. Golemis







21. Crystallographic Studies of the Cerebral Cavernous Malformations Proteins



Oriana S. Fisher, Xiaofeng Li, Weizhi Liu, Rong Zhang, and Titus J. Boggon







22. Microscopy Techniques to Investigate CCM Pathogenesis



Johnathan Abou-Fadel and Jun Zhang







23. Preparation and Analysis of Protein Extracts to Investigate CCM Pathogenesis



Johnathan Abou-Fadel and Jun Zhang







24. Systems Wide Analysis of CCM Signaling Complex (CSC) Alterations in CCM Deficient Models using Omics Approaches



Johnathan Abou-Fadel and Jun Zhang







25. Study of Molecular Interactions of CCM Proteins by using a GAL4-based Yeast Two-hybrid Screening



Federica Finetti and Lorenza Trabalzini







26. Study of CCM Microvascular Endothelial Phenotype by an in vitro Tubule Differentiation Model



Simona Delle Monache and Saverio Francesco Retta







27. Bidimentional in vitro Angiogenic Assays to Study CCM Pathogenesis: Endothelial Cell Proliferation and Migration



Federica Finetti and Lorenza Trabalzini







28. Measurement of Endothelial Barrier Function in Mouse Models of Cerebral Cavernous Malformations using Intravital Microscopy



Angela J. Glading







29. Immunofluorescence of Cell-Cell and Cell-Extracellular Matrix Adhesive Defects in in vitro Endothelial CCM Model. Juxtacrine Role of Mutant Extracellular Matrix on Wild-Type Endothelial Cells



Sandra Manet, Daphne Vannier, Anne-Pascale Bouin, Justyna Lisowska, Corinne Albiges-Rizo, and Eva Faurobert







30. Detection of p62/SQSTM1 Aggregates in Cellular Models of CCM Disease by Immunofluorescence



Saverio Marchi, Saverio Francesco Retta, and Paolo Pinton







31. Notch Signaling in Familial Cerebral Cavernous Malformations and Immunohistochemical Detection of cleaved Notch1 Intracellular Domain



Sana S. Hasan and Andreas Fischer







32. Measuring the Kinase Activity of GCKIII Proteins in vitro



Juan Zalvide, Cristina Almenglo, Sara Vazquez, Mar Garcia-Colomer, Miriam Sartages, and Celia M. Pombo







33. Spectrophotometric Method for Determining Glyoxalase 1 Activity in Cerebral Cavernous Malformation (CCM) Disease



Cinzia Antognelli, Vincenzo Nicola Talesa, and Saverio Francesco Retta







34. Fluorescence Analysis of Reactive Oxygen Species (ROS) in Cellular Models of Cerebral Cavernous Malformation Disease



Andrea Perrelli and Saverio Francesco Retta







35. Small RNA Library Preparation for Profiling Small Non-coding RNAs in Patients Affected by Cavernous Malformations



Souvik Kar, Robert Geffers, Amir Samii, and Helmut Bertalanffy







36. Affinity Purification and Preparation of Peptides for Mass Spectrometry from C. elegans



Evelyn Popiel and William Brent Derry

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