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Buchrieser, Carmen. Hilbi, Hubert.

Legionella : methods and protocols / edited by Carmen Buchrieser, Hubert Hilbi.

2nd ed.

New York :   Humana Press :   Springer,   c2019.  

xxvi, 476 p. : ill. (some col.) ; 27 cm.

Methods in molecular biology,1064-3745 ; 1921

9781493990474 (print) 9781493990481 (eBook) 1493990489 (eBook)

Analysis of iron requirements and siderophore production / Denise M. Burnside and Nicholas P. Cianciotto -- Pathometabolism of Legionella studied by isotopologue profiling / Klaus Heuner, Mareike Kunze, Fan Chen, and Wolfgang Eisenreich -- Quantification of viable but non-culturable cells of Legionella pneumophila / Rosalie S. Braun, Nilmini Mendis, Laam Li, and Sebastien P. Faucher -- Selection of legionella virulence-related traits by environmental protozoa / Francisco Amaro and Howard Shuman -- Migration of Acanthamoeba castellanii through legionella biofilms / Ramon Hochstrasser and Hubert Hilbi -- Scar-free genome editing in Legionella pneumophila / Nathalie Bailo, Hussein Kanaan, Elisabeth Kay, Xavier Charpentier, Patricia Doublet, and Christophe Gilbert -- Querying legionella genomes using transposition-sequencing / Le´o Hardy and Xavier Charpentier -- Screening targeted Legionella pneumophila mutant libraries in vivo using INSeq / Stephanie R. Shames -- Genetic manipulation of non-pneumophila Legionella : protocols developed for Legionella longbeachae / Elizabeth Korevaar, Chen Ai Khoo, and Hayley J. Newton -- Quantitative imaging flow cytometry of Legionella-containing vacuoles in dually fluorescence-labeled Dictyostelium / Amanda Welin, Stephen Weber, and Hubert Hilbi -- Sorting of phagocytic cells infected with Legionella pneumophila / Monica Rolando and Carmen Buchrieser -- Single cell analysis of Legionella and Legionella-infected Acanthamoeba by agarose embedment / Nicolas Personnic, Bianca Striednig, and Hubert Hilbi -- Mitochondrial dynamics and activity in Legionella-infected cells / Ok-Ryul Song, Priscille Brodin, Carmen Buchrieser, and Pedro Escoll -- Perturbation of Legionella cell infection by RNA interference / Bernhard Steiner, A. Leoni Swart, and Hubert Hilbi -- Isolation of the Dot/Icm type IV secretion system core complex from Legionella pneumophila / Tomoko Kubori and Hiroki Nagai -- In situ imaging and structure determination of bacterial toxin delivery systems using electron cryotomography / Debnath Ghosal, Mohammed Kaplan, Yi-Wei Chang, and Grant J. Jensen -- Methods for noncanonical ubiquitination and deubiquitination catalyzed by Legionella pneumophila effector proteins / Jiazhang Qiu and Zhao-Qing Luo -- Purification and analysis of effector glucosyltransferase Lgt1 from Legionella pneumophila / Nadezhda Levanova, Irina Tabakova, Thomas Jank, and Yury Belyi -- Determination of in vivo interactomes of Dot/Icm type IV secretion system effectors by tandem affinity purification / Ernest C. So, Aure´lie Mousnier, Gad Frankel, and Gunnar N. Schroeder -- Inflammasome activation in Legionella-infected macrophages / Danielle P. A. Mascarenhas and Dario S. Zamboni -- Infection of human lung tissue explants (HLTEs) with Legionella pneumophila / Lina Scheithauer and Michael Steinert -- Galleria mellonella infection model for investigating the molecular mechanisms of Legionella virulence / Gad Frankel and Gunnar N. Schroeder -- Dictyostelium host response to legionella infection : strategies and assays / Salvatore Bozzaro, Simona Buracco, Barbara Peracino, and Ludwig Eichinger -- Caenorhabditis elegans model of Legionella infection / Ann Karen C. Brassinga and Costi D. Sifri -- Mouse as a model for pulmonary Legionella infection / Garrett Z. Ng, Anastasia Solomatina, Ian R. van Driel, and Elizabeth L. Hartland -- Targeted metagenomics of microbial diversity in free-living amoebae and water samples / Vincent Delafont, Yoann Perrin, Didier Bouchon, Laurent Moulin, and Yann He´chard -- Analysis of the pulmonary microbiome composition of Legionella pneumophila-infected patients / Ana Elena Pe´rez-Cobas and Carmen Buchrieser -- Subcellular protein fractionation in Legionella pneumophila and preparation of the derived sub-proteomes for analysis by mass spectrometry / Sandra Maaß, Gina Moog, and Do¨rte Becher.

Includes bibliographical references and index.

Legionella. Legionnaires' disease. Legionella. Legionnaires' Disease.