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Influenza virus : methods and protocols

Influenza virus : methods and protocols (1회 대출)

자료유형
단행본
개인저자
Yamauchi, Yohei.
서명 / 저자사항
Influenza virus : methods and protocols / edited by Yohei Yamauchi.
발행사항
New York :   Humana Press :   Springer,   c2018.  
형태사항
1 online resource (xvi, 663 p.) : ill. (some col.) ; 27 cm.
총서사항
Springer protocols
ISBN
9781493986774
요약
This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions. Chapters detail a broad range of methods from diagnosis, virus propagation, proteomics, haploid screening, lentiviral screening, virus entry, single molecule RNA imaging, correlative light and electron microscopy (CLEM), EM, light-sheet microscopy, biochemistry, viral transcription, physiological infection models, animal models, in vivo imaging, antigenic evolution, immunology to mathematical modelling. Reviews cover general influenza, clinical trials, both sides of the gain-of-function debate, and computational modelling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Influenza Virus: Methods and Protocols aims to motivate experienced researchers and newcomers in the field and improve our overall understanding of influenza.
내용주기
Understanding Influenza -- Clinical Diagnosis of Influenza -- Influenza A Virus Genetic Tools -- From Clinical Sample to Molecular Clone -- Propagation and Titration of Influenza Viruses -- Purification and Proteomics of Influenza Virions -- Haploid Screening for the Identification of Host Factors in Virus Infection -- Phenotypic Lentivirus Screens to Identify Antiviral Single Domain Antibodies -- Deciphering Virus Entry with Fluorescently-Labeled Viral Particles -- Quantitative RT-PCR Analysis of Influenza Virus Endocytic Escape -- Single Molecule Sensitivity RNA FISH Analysis of Influenza Virus Genome Trafficking -- Correlative Light and Electron Microscopy (CLEM) and 3D Methods to Study Architecture of Virus-Infected Cells -- Correlative Light and Electron Microscopy (CLEM) of Influenza Virus Entry and Budding -- Influenza Virus-Liposome Fusion Studies using Fluorescence Dequenching and Cryo-Electron Tomography -- Metal-Tagging Transmission Electron Microscopy and Immunogold Labelling on Tokuyasu Cryosections to Image Influenza A Virus Ribonucleoprotein Transport and Packaging -- Live Imaging of Influenza Viral Ribonucleoproteins using Light-Sheet Microscopy -- Purification of Unanchored Polyubiquitin Chains from Influenza Virions -- Measurement of Influenza Virus Polymerase Activity -- In vitro models to study influenza virus and Staphylococcus aureus super-infection on a molecular level -- Infection of Cultured Mammalian Cells with Aerosolized Influenza Virus -- Animal Models in Influenza Research -- Measuring Influenza Virus Infection using Bioluminescent Reporter Viruses for in vivo Imaging and in vitro Replication Assays -- Selection of Antigenically Advanced Variants of Influenza Viruses -- Assessment of Influenza Virus Hemagglutinin Stalk-specific Antibody Responses -- Analyses of Cellular Immune Responses from Ferrets Following Influenza Virus Infection -- Parameter Estimation in Mathematical Models of Viral Infections using R -- Software for Characterizing the Antigenic and Genetic Evolution of Human Influenza Viruses -- Clinical Trials of Influenza Vaccines -- Special Challenges -- The Silver Lining in Gain-of-Function Experiments with Pathogens of Pandemic Potential -- Why Do Exceptionally Dangerous Gain-of-Function Experiments in Influenza? -- How Computational Models Enable Mechanistic Insights into Virus Infection.
서지주기
Includes bibliographical references and index.
일반주제명
Emerging infectious diseases.
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082 0 4 ▼a 616.203 ▼2 23
084 ▼a 616.203 ▼2 DDCK
090 ▼a 616.203 ▼b I432
245 0 0 ▼a Influenza virus : ▼b methods and protocols / ▼c edited by Yohei Yamauchi.
260 ▼a New York : ▼b Humana Press : ▼b Springer, ▼c c2018.
300 ▼a 1 online resource (xvi, 663 p.) : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Springer protocols
490 1 ▼a Methods in Molecular Biology, ▼x 1064-3745 ; ▼v 1836
504 ▼a Includes bibliographical references and index.
505 0 ▼a Understanding Influenza -- Clinical Diagnosis of Influenza -- Influenza A Virus Genetic Tools -- From Clinical Sample to Molecular Clone -- Propagation and Titration of Influenza Viruses -- Purification and Proteomics of Influenza Virions -- Haploid Screening for the Identification of Host Factors in Virus Infection -- Phenotypic Lentivirus Screens to Identify Antiviral Single Domain Antibodies -- Deciphering Virus Entry with Fluorescently-Labeled Viral Particles -- Quantitative RT-PCR Analysis of Influenza Virus Endocytic Escape -- Single Molecule Sensitivity RNA FISH Analysis of Influenza Virus Genome Trafficking -- Correlative Light and Electron Microscopy (CLEM) and 3D Methods to Study Architecture of Virus-Infected Cells -- Correlative Light and Electron Microscopy (CLEM) of Influenza Virus Entry and Budding -- Influenza Virus-Liposome Fusion Studies using Fluorescence Dequenching and Cryo-Electron Tomography -- Metal-Tagging Transmission Electron Microscopy and Immunogold Labelling on Tokuyasu Cryosections to Image Influenza A Virus Ribonucleoprotein Transport and Packaging -- Live Imaging of Influenza Viral Ribonucleoproteins using Light-Sheet Microscopy -- Purification of Unanchored Polyubiquitin Chains from Influenza Virions -- Measurement of Influenza Virus Polymerase Activity -- In vitro models to study influenza virus and Staphylococcus aureus super-infection on a molecular level -- Infection of Cultured Mammalian Cells with Aerosolized Influenza Virus -- Animal Models in Influenza Research -- Measuring Influenza Virus Infection using Bioluminescent Reporter Viruses for in vivo Imaging and in vitro Replication Assays -- Selection of Antigenically Advanced Variants of Influenza Viruses -- Assessment of Influenza Virus Hemagglutinin Stalk-specific Antibody Responses -- Analyses of Cellular Immune Responses from Ferrets Following Influenza Virus Infection -- Parameter Estimation in Mathematical Models of Viral Infections using R -- Software for Characterizing the Antigenic and Genetic Evolution of Human Influenza Viruses -- Clinical Trials of Influenza Vaccines -- Special Challenges -- The Silver Lining in Gain-of-Function Experiments with Pathogens of Pandemic Potential -- Why Do Exceptionally Dangerous Gain-of-Function Experiments in Influenza? -- How Computational Models Enable Mechanistic Insights into Virus Infection.
520 ▼a This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions. Chapters detail a broad range of methods from diagnosis, virus propagation, proteomics, haploid screening, lentiviral screening, virus entry, single molecule RNA imaging, correlative light and electron microscopy (CLEM), EM, light-sheet microscopy, biochemistry, viral transcription, physiological infection models, animal models, in vivo imaging, antigenic evolution, immunology to mathematical modelling. Reviews cover general influenza, clinical trials, both sides of the gain-of-function debate, and computational modelling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Influenza Virus: Methods and Protocols aims to motivate experienced researchers and newcomers in the field and improve our overall understanding of influenza.
650 0 ▼a Emerging infectious diseases.
700 1 ▼a Yamauchi, Yohei.
830 0 ▼a Springer protocols.
830 0 ▼a Methods in molecular biology ; ▼v 1836.
945 ▼a KLPA

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 616.203 I432 등록번호 121246495 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

목차

Understanding Influenza
Clinical Diagnosis of Influenza
Influenza A Virus Genetic Tools - From Clinical Sample to Molecular Clone
Propagation and Titration of Influenza Viruses
Purification and Proteomics of Influenza Virions
Haploid Screening for the Identification of Host Factors in Virus Infection
Phenotypic Lentivirus Screens to Identify Antiviral Single Domain Antibodies
Deciphering Virus Entry with Fluorescently-Labeled Viral Particles
Quantitative RT-PCR Analysis of Influenza Virus Endocytic Escape
Single Molecule Sensitivity RNA FISH Analysis of Influenza Virus Genome Trafficking
Correlative Light and Electron Microscopy (CLEM) and 3D Methods to Study Architecture of Virus-Infected Cells
Correlative Light and Electron Microscopy (CLEM) of Influenza Virus Entry and Budding
Influenza Virus-Liposome Fusion Studies using Fluorescence Dequenching and Cryo-Electron Tomography
Metal-Tagging Transmission Electron Microscopy and Immunogold Labelling on Tokuyasu Cryosections to Image Influenza A Virus Ribonucleoprotein Transport and Packaging
Live Imaging of Influenza Viral Ribonucleoproteins using Light-Sheet Microscopy
Purification of Unanchored Polyubiquitin Chains from Influenza Virions
Measurement of Influenza Virus Polymerase Activity
In vitro models to study influenza virus and Staphylococcus aureus super-infection on a molecular level
Infection of Cultured Mammalian Cells with Aerosolized Influenza Virus
Animal Models in Influenza Research
Measuring Influenza Virus Infection using Bioluminescent Reporter Viruses for in vivo Imaging and in vitro Replication Assays
Selection of Antigenically Advanced Variants of Influenza Viruses
Assessment of Influenza Virus Hemagglutinin Stalk-specific Antibody Responses
Analyses of Cellular Immune Responses from Ferrets Following Influenza Virus Infection
Parameter Estimation in Mathematical Models of Viral Infections using R
Software for Characterizing the Antigenic and Genetic Evolution of Human Influenza Viruses
Clinical Trials of Influenza Vaccines – Special Challenges
The Silver Lining in Gain-of-Function Experiments with Pathogens of Pandemic Potential
Why Do Exceptionally Dangerous Gain-of-Function Experiments in Influenza?
How Computational Models Enable Mechanistic Insights into Virus Infection.

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