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Type-1 diabetes : methods and protocols

Type-1 diabetes : methods and protocols (Loan 1 times)

Material type
단행본
Personal Author
Gillespie, Kathleen M.
Title Statement
Type-1 diabetes : methods and protocols / edited by Kathleen M. Gillespie.
Publication, Distribution, etc
New York, NY :   Humana Press :   Springer,   c2016.  
Physical Medium
x, 213 p. : ill. (some color) ; 27 cm.
Series Statement
Springer protocols
ISBN
9781493936410 (print) 9781493936434 (electronic bk.)
요약
The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.
Content Notes
Type 1 diabetes: Current perspectives -- Type 1 diabetes high-risk HLA class ii determination by polymerase chain reaction sequence-specific primers -- Pancreatic beta cell survival and signaling pathways: Effects of type 1 diabetes-associated genetic variants -- Islet autoantibody analysis: Radioimmunoassays -- Islet autoantibody detection by electrochemiluminescence (ECL) assay -- Detection of C-peptide in urine as a measure of ongoing beta cell function -- Histology of type 1 diabetes pancreas -- Identification of islet antigen-specific CD8 T cells using mhci-peptide tetramer reagents in the non obese diabetic (NOD) mouse model of type 1 diabetes -- Tracking immunological responses of islet antigen-specific T cells in the nonobese diabetic (NOD) mouse model of type 1 diabetes -- Adoptive transfer of autoimmune diabetes using immunodeficient nonobese diabetic (NOD) mice -- Methylation analysis in distinct immune cell subsets in type 1 diabetes -- Fluorescence in situ hybridization with concomitant immunofluorescence in human pancreas -- Laser capture and single cell genotyping from frozen tissue sections -- The gut microbiome in the NOD mouse -- Molecular methods and protein synthesis for definition of autoantibody epitopes.
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Diabetes Mellitus, Type 1. Diabetes.
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020 ▼a 9781493936410 (print)
020 ▼a 9781493936434 (electronic bk.)
035 ▼a (OCoLC)951536404
040 ▼a GW5XE ▼b eng ▼e rda ▼e pn ▼c GW5XE ▼d TMC ▼d YDXCP ▼d OCLCF ▼d SCB ▼d OCLCO ▼d AZU ▼d KUB ▼d 211009
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082 0 4 ▼a 616.4/622 ▼2 23
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090 ▼a 616.4622 ▼b T991
245 0 0 ▼a Type-1 diabetes : ▼b methods and protocols / ▼c edited by Kathleen M. Gillespie.
246 3 ▼a Type 1 diabetes
246 3 ▼a Type one diabetes
260 ▼a New York, NY : ▼b Humana Press : ▼b Springer, ▼c c2016.
300 ▼a x, 213 p. : ▼b ill. (some color) ; ▼c 27 cm.
490 1 ▼a Springer protocols
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 1433
504 ▼a Includes bibliographical references and index.
505 0 0 ▼t Type 1 diabetes: Current perspectives -- ▼t Type 1 diabetes high-risk HLA class ii determination by polymerase chain reaction sequence-specific primers -- ▼t Pancreatic beta cell survival and signaling pathways: Effects of type 1 diabetes-associated genetic variants -- ▼t Islet autoantibody analysis: Radioimmunoassays -- ▼t Islet autoantibody detection by electrochemiluminescence (ECL) assay -- ▼t Detection of C-peptide in urine as a measure of ongoing beta cell function -- ▼t Histology of type 1 diabetes pancreas -- ▼t Identification of islet antigen-specific CD8 T cells using mhci-peptide tetramer reagents in the non obese diabetic (NOD) mouse model of type 1 diabetes -- ▼t Tracking immunological responses of islet antigen-specific T cells in the nonobese diabetic (NOD) mouse model of type 1 diabetes -- ▼t Adoptive transfer of autoimmune diabetes using immunodeficient nonobese diabetic (NOD) mice -- ▼t Methylation analysis in distinct immune cell subsets in type 1 diabetes -- ▼t Fluorescence in situ hybridization with concomitant immunofluorescence in human pancreas -- ▼t Laser capture and single cell genotyping from frozen tissue sections -- ▼t The gut microbiome in the NOD mouse -- ▼t Molecular methods and protein synthesis for definition of autoantibody epitopes.
520 ▼a The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.
650 1 2 ▼a Diabetes Mellitus, Type 1.
650 0 ▼a Diabetes.
700 1 ▼a Gillespie, Kathleen M.
830 0 ▼a Springer protocols.
830 0 ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v v. 1433.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 616.4622 T991 Accession No. 121237406 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

Type 1 Diabetes: Prediction And Prevention: Current Perspectives.- The HLA.- Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants.- Islet Autoantibody Analysis: Radioimmunoassays.- Islet Autoantibody Analysis: Non-Radioactive Assays - Electrochemiluminescence.- Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function in Type 1 Diabetes.- Histology of the Type 1 Diabetes Pancreas.- Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.- Tracking Immunological Responses of Islet Antigen-Specific T Cells In The Non Obese Diabetic (NOD) Mouse Model Of Type 1 Diabetes.- Adoptive Transfer of Autoimmune Diabetes using Immunodeficient Non Obese Diabetic (NOD) Mice.- Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes.- FISH with Concomitant Immunofluorescence.- Laser Capture Microdissection: Single Pancreatic Cell DNA Profiling.- The Gut Microbiome in the NOD Mouse.- Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes.


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