CONTENTS
Preface = v
Contributors = ix
PART Ⅰ DETECTION OF MOLECULAR MARKERS OF BREAST CANCER
1 Basic Histopathological Methods and Breast Lesion Types for Research / Nengtai Ouyang ; Lin Wang = 3
2 Clinical Applications for Immunohistochemistry of Breast Lesions / Kester Haye ; Rajarsi Gupta ; Christopher Metter ; Jingxuan Liu = 11
3 Immunohistochemistry for Triple-Negative Breast Cancer / Kalnisha Naidoo ; Sarah E. Pinder = 39
4 In Situ Hybridization of Breast Cancer Markers / Li Min ; Chengchao Shou = 53
5 Evaluation of Human Epidermal Growth Factor Receptor 2(HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin-Embedded(FFPE) Tissue Specimens by Fluorescence In Situ Hybridization(FISH) / Harry C. Hwang ; Allen M. Gown = 61
PART Ⅱ GENETIC DETECTION FOR BREAST CANCER
6 Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction(PCR) / Yiyi Li ; Kai Wang ; Longhua Chen ; Xiaoxia Zhu ; Jie Zhou = 73
7 Detection of miRNA in Cultured Cells or Xenograft Tissues of Breast Cancer / Martin Brown ; Meiyun Fan = 81
8 Pyrosequencing Analysis for Breast Cancer DNA Methylome / Cent Kuscu ; Canan Kuscu = 89
PART Ⅲ ISOLATION OF BREAST CANCER CELLS
9 Vita-Assay™ Method of Enrichment and Identification of Circulating Cancer Cells／Circulating Tumor Cells(CTCs) / Shaun Tulley ; Qiang Zhao ; Huan Dong ; Michael L. Pearl ; Wen-Tien Chen = 107
10 Breast Cancer Stem Cell Isolation / Xuanmao Jiao ; Albert A. Rizvanov ; Massimo Cristofanilli ; Regina R. Miftakhova ; Richard G. Pestell = 121
PART Ⅳ IN VITRO EXPERIMENTAL MODELS FOR BREAST CANCER
11 Cellular Apoptosis Assay of Breast Cancer / Yu Sun ; Wei-Xing Zong = 139
12 Assessment of Matrix Metalloproteinases by Gelatin Zymography / Jillian Cathcart = 151
13 Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay / Ty J. Lively ; Dale B. Bosco ; Zahraa I. Khamis ; Qing-Xiang Amy Sang = 161
14 Determination of Breast Cancer Cell Migratory Ability / David Schmitt ; Joel Andrews ; Ming Tan = 171
15 A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration / Vincent M. Alford ; Eric Roth ; Qian Zhang ; Jian Cao = 181
16 Three-Dimensional Assay for Studying Breast Cancer Cell Invasion / Nikki A. Evensen = 189
17 A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation / Ashleigh Pulkoski-Gross = 195
18 Analysis of Invadopodia Formation in Breast Cancer Cells / Ziqing Wang ; Xiao Liang ; Ming Cai ; Guangwei Du = 203
19 Patient-Derived Tumor Xenograft Models of Breast Cancer / Christopher D. Suarez ; Laurie E. Littlepage = 211
20 Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors / Maryia Lu ; Li Wei Rachel Tay ; Jingquan He ; Guangwei Du = 225
21 3D In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method / Fransisca Leonard ; Biana Godin = 239
PART Ⅴ IN VIVO EXPERIMENTAL MODELS FOR BREAST CANCER
22 Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model / Jacquelyn J. Ames ; Terry Henderson ; Lucy Liaw ; Peter C. Brooks = 255
23 Pharmacokinetics and Pharmacodynamics in Breast Cancer Animal Models / Wei Wang ; Subhasree Nag ; Ruiwen Zhang = 271
24 Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells / Derek Rammelkamp ; Weiyi Li ; Yizhi Meng = 289
25 Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression / Gregg B. Fields ; Maciej J. Stawikowski = 303
Index = 331
