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Estrogen receptors : methods and protocols

Estrogen receptors : methods and protocols

Material type
단행본
Personal Author
Eyster, Kathleen M.
Title Statement
Estrogen receptors : methods and protocols / edited by Kathleen M. Eyster.
Publication, Distribution, etc
New York :   Humana Press :   Springer,   c2016.  
Physical Medium
xvi, 576 p. : ill. (some col.) ; 27 cm.
Series Statement
Methods in molecular biology ;1366
ISBN
9781493931262 9781493931279 (ebk.) 149393127X (ebk.)
요약
This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen. .
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Estrogens. Receptors, Estrogen --therapeutic use. Estrogen Receptor Modulators. Life sciences. Endocrinology. Biochemistry.
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020 ▼a 9781493931262
020 ▼a 9781493931279 (ebk.)
020 ▼a 149393127X (ebk.)
035 ▼a (OCoLC)930636321
040 ▼a RML ▼b eng ▼c RML ▼d RML ▼d OCLCO ▼d OCLCQ ▼d AZU ▼d OCLCO ▼d OCLCF ▼d KUB ▼d 211009
050 4 ▼a QD415-436
082 0 4 ▼a 612.405 ▼2 23
084 ▼a 612.405 ▼2 DDCK
090 ▼a 612.405 ▼b E82
245 0 0 ▼a Estrogen receptors : ▼b methods and protocols / ▼c edited by Kathleen M. Eyster.
260 ▼a New York : ▼b Humana Press : ▼b Springer, ▼c c2016.
300 ▼a xvi, 576 p. : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Methods in molecular biology ; ▼v 1366
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
520 ▼a This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen. .
650 2 ▼a Estrogens.
650 2 ▼a Receptors, Estrogen ▼x therapeutic use.
650 2 ▼a Estrogen Receptor Modulators.
650 0 ▼a Life sciences.
650 0 ▼a Endocrinology.
650 0 ▼a Biochemistry.
700 1 ▼a Eyster, Kathleen M.
830 0 ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v v. 1366.
830 0 ▼a Springer protocols (Series).
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 612.405 E82 Accession No. 121235762 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

1 . The Estrogen Receptors: An Overview from Different Perspectives Kathleen M. Eyster 2 . Competitive Binding Assay for the G-Protein Coupled Receptor 30 (GPR30) or G-Protein Coupled Estrogen Receptor (GPER) Thomas Thekkumkara, Russell Snyder, and Vardan T. Karamyan 3 . The Use of Real Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression Michelle L. Booze and Kathleen M. Eyster 4. Bioinformatics Analysis of Estrogen-Responsive Genes Adam E. Handel 5. Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen Kwi Hye Koh and Hyunyoung Jeong 6. Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors Meike Wagner, Johannes Jung, Michael Koslowski, Ozlem Tureci, Vijay K. Tiwari, and Ugur Sahin 7. Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells Jing Lin and Zhenmin Lei 8. Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer Kelly A. Holmes, Gordon D. Brown, and Jason S. Carroll 9. RNA-Seq Experiment and Data Analysis Hanquan Liang and Erliang Zeng 10. DNA Microarray Analysis of Estrogen-Responsive Genes Kathleen M. Eyster 11. Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two Dimensional Liquid Chromatography and Tandem Mass Spectrometry Eduardo A. Callegari 12. Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Placenta, and Fetal Adrenal in Response to Estrogen Thomas W. Bonagura, Jeffery S. Babischkin, Gerald J. Pepe and Eugene D. Albrecht 13. Co-Localization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy Araceli Morales, Raquel Marin, Jorge Marrero-Alonso, Alicia Boto, and Mario Diaz 14. Live Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy Kassandra Kisler and Reymundo Dominguez 15. In Situ Hybridization of Estrogen Receptors and and GPER in the Human Testis D. Fietz, M. Bergmann, and K. Hartmann 16. Purification of Histone Lysine Methyltransferase SMYD2 and Co-Crystallization with a Target Peptide from Estrogen Receptor a Yuanyuan Jiang, Joshua Holcomb, Nicholas Spellmon, and Zhe Yang 17. Gold Nanoparticle-Based Forster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA Interaction Xiaodi Su, Khin Moh Moh Aung, Steven Lukman, and Bin Liu 18. Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy M.K. Thakur and V. Paramanik 19. Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction between the Estrogen Receptor Alpha and Co-Activators: The Case of Calmodulin Emeric Miclet, Sandrine Bourgoin-Voillard, Cillian Byrne, and Yves Jacquot 20. Surface Plasmon Resonance Study of Cooperative Interactions of Estrogen Receptor and Specificity Protein 1 with Composite DNA Elements Xiaodi Su and Hong Yan Song 21. Tips and Tricks for Successful Application of Statistical Methods to Biological Data Evelyn Schlenker 22. The Synonymous Ala87 Mutation of Estrogen Receptor Alpha Modifies Transcriptional Activation through Both ERE and AP1 Sites Tamara Fernandez-Calero, Gilles Flouriot, and Monica Marin 23. Selective Estrogen Receptor Modulators and the Tissue-Selective Estrogen Complex: Analysis of Cell Type-Specific Effects using In Vivo Imaging of a Reporter Mouse Model Sara Della Torre and Paolo Ciana 24. Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model Daniel A. Gorelick, Caroline Lucia Pinto, Ruixin Hao, and Maria Bondesson 25. Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo Culture Natalija Eigeliene, Risto Erkkola, and Pirk 1 . The Estrogen Receptors: An Overview from Different Perspectives Kathleen M. Eyster 2 . Competitive Binding Assay for the G-Protein Coupled Receptor 30 (GPR30) or G-Protein Coupled Estrogen Receptor (GPER) Thomas Thekkumkara, Russell Snyder, and Vardan T. Karamyan 3 . The Use of Real Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression Michelle L. Booze and Kathleen M. Eyster 4. Bioinformatics Analysis of Estrogen-Responsive Genes Adam E. Handel 5. Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen Kwi Hye Koh and Hyunyoung Jeong 6. Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors Meike Wagner, Johannes Jung, Michael Koslowski, Ozlem Tureci, Vijay K. Tiwari, and Ugur Sahin 7. Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells Jing Lin and Zhenmin Lei 8. Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer Kelly A. Holmes, Gordon D. Brown, and Jason S. Carroll 9. RNA-Seq Experiment and Data Analysis Hanquan Liang and Erliang Zeng 10. DNA Microarray Analysis of Estrogen-Responsive Genes Kathleen M. Eyster 11. Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two Dimensional Liquid Chromatography and Tandem Mass Spectrometry Eduardo A. Callegari 12. Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Placenta, and Fetal Adrenal in Response to Estrogen Thomas W. Bonagura, Jeffery S. Babischkin, Gerald J. Pepe and Eugene D. Albrecht 13. Co-Localization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy Araceli Morales, Raquel Marin, Jorge Marrero-Alonso, Alicia Boto, and Mario Diaz 14. Live Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy Kassandra Kisler and Reymundo Dominguez 15. In Situ Hybridization of Estrogen Receptors and beta and GPER in the Human Testis D. Fietz, M. Bergmann, and K. Hartmann 16. Purification of Histone Lysine Methyltransferase SMYD2 and Co-Crystallization with a Target Peptide from Estrogen Receptor a Yuanyuan Jiang, Joshua Holcomb, Nicholas Spellmon, and Zhe Yang 17. Gold Nanoparticle-Based Forster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA Interaction Xiaodi Su, Khin Moh Moh Aung, Steven Lukman, and Bin Liu 18. Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy M.K. Thakur and V. Paramanik 19. Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction between the Estrogen Receptor Alpha and Co-Activators: The Case of Calmodulin Emeric Miclet, Sandrine Bourgoin-Voillard, Cillian Byrne, and Yves Jacquot 20. Surface Plasmon Resonance Study of Cooperative Interactions of Estrogen Receptor and Specificity Protein 1 with Composite DNA Elements Xiaodi Su and Hong Yan Song 21. Tips and Tricks for Successful Application of Statistical Methods to Biological Data Evelyn Schlenker 22. The Synonymous Ala87 Mutation of Estrogen Receptor Alpha Modifies Transcriptional Activation through Both ERE and AP1 Sites Tamara Fernandez-Calero, Gilles Flouriot, and Monica Marin Selective Estrogen Receptor Modulators and the Tissue-Selective Estrogen Complex: Analysis of Cell Type-Specific Effects using In Vivo Imaging of a Reporter Mouse Model Sara Della Torre and Paolo Ciana 24. Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model Daniel A. Gorelick, Caroline Lucia Pinto, Ruixin Hao, and Maria Bondesson 25. Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo Culture Natalija Eigeliene, Ris


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