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Notch signaling : methods and protocols

Notch signaling : methods and protocols (4회 대출)

자료유형
단행본
개인저자
Bellen, Hugo J. Yamamoto, Shinya.
서명 / 저자사항
Notch signaling : methods and protocols / edited by Hugo J. Bellen, Department of Molecular and Human Genetics, Program in Developmental Biology, Department of Neuroscience, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA, Shinya Yamamoto, Department of Molecular and Human Genetics, Program in Developmental Biology, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA.
발행사항
New York :   Humana Press :   Springer,   2014.  
형태사항
xi, 351 p. : ill. (some col.) ; 26 cm.
총서사항
Methods in molecular biology,1064-3745 ; 1187
ISBN
9781493911387 (alk. paper) 1493911384 (alk. paper)
내용주기
Introduction to notch signaling / Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen -- Genetic screens to identify new notch pathway mutants in Drosophila / Nikolaos Giagtzoglou -- Structure-function analysis of Drosophila notch using genomic tescue transgenes / Jessica Leonardi and Hamed Jafar-Nejad -- Overview of genetic tools and techniques to study notch signaling in mice / Thomas Gridley and Andrew K. Groves -- Immunohistochemical tools and techniques to visualize notch in Drosophila melanogaster / Emiliana Tognon and Thomas Vaccari -- Antibody uptake assay and in vivo imaging to study intracellular trafficking of notch and delta in Drosophila / Lydie Couturier and François Schweisguth -- Tracking trafficking of notch and its ligands in mammalian cells / Patricia Chastagner and Christel Brou -- Visualizing notch signaling in vivo in Drosophila tissues / Benjamin E. Housden, Jinghua Li, and Sarah J. Bray -- Monitoring notch activity in the mouse / Swananda Marathe and Lavinia Alberi -- Notch signaling assays in Drosophila cultured cell lines / Jinghua Li, Benjamin E. Housden, and Sarah J. Bray -- Monitoring notch activation in cultured mammalian cells : transcriptional reporter assays / Ma. Xenia G. Ilagan and Raphael Kopan -- Monitoring notch activation in cultured mammalian cells : luciferase complementation imaging assays / Ma. Xenia G. Ilagan and Raphael Kopan -- Visualization of notch signaling oscillation in cells and tissues / Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama -- Proteomic analysis of the notch interactome / K. G. Guruharsha ... [et al.] -- Bacterial expression and in vitro refolding of limited fragments of the notch receptor and its ligands / Pat Whiteman, Christina Redfield, and Penny A. Handford -- Analyzing the posttranslational modification status of notch using mass spectrometry / Shinako Kakuda and Robert S. Haltiwanger -- Assay to probe proteolytic processing of notch by [gamma]-secretase / Lutgarde Serneels, Ina Tesseur, and Bart De Strooper -- Analyzing the nuclear complexes of notch signaling by electrophoretic mobility shift assay / Kelly L. Arnett and Stephen C. Blacklow -- Identifying direct notch transcriptional targets using the GSI-washout assay / Will Bailis, Yumi Yashiro-Ohtani, and Warren S. Pear -- Probing the epigenetic status at notch target genes / Robert Liefke and Tilman Borggrefe -- Notch-ligand binding assays in Drosophila cells / Aiguo Xu and Kenneth D. Irvine -- Modeling notch signaling : a practical tutorial / Pau Formosa-Jordan and David Sprinzak -- Small molecules that inhibit notch signaling / Gerdien E. De Kloe and Bart De Strooper -- Application and evaluation of anti-notch antibodies to modulate notch signaling / Wendy R. Gordon and Jon C. Aster -- Application of anti-ligand antibodies to inhibit notch signaling / Jun-ichiro Koga and Masanori Aikawa.
서지주기
Includes bibliographical references and index.
일반주제명
Notch genes --Laboratory manuals. Notch proteins --Laboratory manuals. Receptors, Notch --Laboratory Manuals. Signal Transduction --Laboratory Manuals.
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020 ▼a 9781493911387 (alk. paper)
020 ▼a 1493911384 (alk. paper)
020 ▼z 9781493911394 (eBook)
020 ▼z 1493911392 (eBook)
035 ▼a (KERIS)REF000017806609
040 ▼a NLM ▼b eng ▼c NLM ▼e rda ▼d YDXCP ▼d BTCTA ▼d UKMGB ▼d HNK ▼d FTU ▼d NUI ▼d OCLCF ▼d OCLCO ▼d DLC ▼d 211009
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082 0 0 ▼a 616.8/31071 ▼2 23
084 ▼a 616.831071 ▼2 DDCK
090 ▼a 616.831071 ▼b N899
130 0 ▼a Notch signaling (Bellen)
245 1 0 ▼a Notch signaling : ▼b methods and protocols / ▼c edited by Hugo J. Bellen, Department of Molecular and Human Genetics, Program in Developmental Biology, Department of Neuroscience, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA, Shinya Yamamoto, Department of Molecular and Human Genetics, Program in Developmental Biology, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA.
260 ▼a New York : ▼b Humana Press : ▼b Springer, ▼c 2014.
300 ▼a xi, 351 p. : ▼b ill. (some col.) ; ▼c 26 cm.
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 1187
490 1 ▼a Springer protocols, ▼x 1949-2448
504 ▼a Includes bibliographical references and index.
505 0 ▼t Introduction to notch signaling / ▼r Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen -- ▼t Genetic screens to identify new notch pathway mutants in Drosophila / ▼r Nikolaos Giagtzoglou -- ▼t Structure-function analysis of Drosophila notch using genomic tescue transgenes / ▼r Jessica Leonardi and Hamed Jafar-Nejad -- ▼t Overview of genetic tools and techniques to study notch signaling in mice / ▼r Thomas Gridley and Andrew K. Groves -- ▼t Immunohistochemical tools and techniques to visualize notch in Drosophila melanogaster / ▼r Emiliana Tognon and Thomas Vaccari -- ▼t Antibody uptake assay and in vivo imaging to study intracellular trafficking of notch and delta in Drosophila / ▼r Lydie Couturier and François Schweisguth -- ▼t Tracking trafficking of notch and its ligands in mammalian cells / ▼r Patricia Chastagner and Christel Brou -- ▼t Visualizing notch signaling in vivo in Drosophila tissues / ▼r Benjamin E. Housden, Jinghua Li, and Sarah J. Bray -- ▼t Monitoring notch activity in the mouse / ▼r Swananda Marathe and Lavinia Alberi -- ▼t Notch signaling assays in Drosophila cultured cell lines / ▼r Jinghua Li, Benjamin E. Housden, and Sarah J. Bray -- ▼t Monitoring notch activation in cultured mammalian cells : transcriptional reporter assays / ▼r Ma. Xenia G. Ilagan and Raphael Kopan -- ▼t Monitoring notch activation in cultured mammalian cells : luciferase complementation imaging assays / ▼r Ma. Xenia G. Ilagan and Raphael Kopan -- ▼t Visualization of notch signaling oscillation in cells and tissues / ▼r Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama -- ▼t Proteomic analysis of the notch interactome / ▼r K. G. Guruharsha ... [et al.] -- ▼t Bacterial expression and in vitro refolding of limited fragments of the notch receptor and its ligands / ▼r Pat Whiteman, Christina Redfield, and Penny A. Handford -- ▼t Analyzing the posttranslational modification status of notch using mass spectrometry / ▼r Shinako Kakuda and Robert S. Haltiwanger -- ▼t Assay to probe proteolytic processing of notch by [gamma]-secretase / ▼r Lutgarde Serneels, Ina Tesseur, and Bart De Strooper -- ▼t Analyzing the nuclear complexes of notch signaling by electrophoretic mobility shift assay / ▼r Kelly L. Arnett and Stephen C. Blacklow -- ▼t Identifying direct notch transcriptional targets using the GSI-washout assay / ▼r Will Bailis, Yumi Yashiro-Ohtani, and Warren S. Pear -- ▼t Probing the epigenetic status at notch target genes / ▼r Robert Liefke and Tilman Borggrefe -- ▼t Notch-ligand binding assays in Drosophila cells / ▼r Aiguo Xu and Kenneth D. Irvine -- ▼t Modeling notch signaling : a practical tutorial / ▼r Pau Formosa-Jordan and David Sprinzak -- ▼t Small molecules that inhibit notch signaling / ▼r Gerdien E. De Kloe and Bart De Strooper -- ▼t Application and evaluation of anti-notch antibodies to modulate notch signaling / ▼r Wendy R. Gordon and Jon C. Aster -- ▼t Application of anti-ligand antibodies to inhibit notch signaling / ▼r Jun-ichiro Koga and Masanori Aikawa.
650 0 ▼a Notch genes ▼v Laboratory manuals.
650 0 ▼a Notch proteins ▼v Laboratory manuals.
650 1 2 ▼a Receptors, Notch ▼v Laboratory Manuals.
650 1 2 ▼a Signal Transduction ▼v Laboratory Manuals.
700 1 ▼a Bellen, Hugo J.
700 1 ▼a Yamamoto, Shinya.
830 0 ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v v. 1187.
830 0 ▼a Springer protocols (Series).
945 ▼a KLPA

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 616.831071 N899 등록번호 121234805 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

목차

1. Introduction to Notch Signaling Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen 2. Genetic Screens to Identify New Notch Pathway Mutants in Drosophila Nikolaos Giagtzoglou 3. Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes Jessica Leonardi and Hamed Jafar-Nejad 4. Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice Thomas Gridley and Andrew K. Groves 5. Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster Emiliana Tognon and Thomas Vaccari 6. Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila Lydie Couturier and Francois Schweisguth 7. Tracking Trafficking of Notch and its Ligands in Mammalian Cells Patricia Chastagner and Christel Brou 8. Visualizing Notch Signaling in vivo in Drosophila Tissues Benjamin E. Housden, Jinghua Li, and Sarah J. Bray 9. Monitoring Notch Activity in the Mouse Lavinia Alberi and Swananda Marathe 10. Notch Signaling Assays in Drosophila Cultured Cell Lines Jinghua Li, Benjamin E. Housden, and Sarah J. Bray 11. Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays Ma. Xenia G. Ilagan and Raphael Kopan 12. Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays Ma. Xenia G. Ilagan and Raphael Kopan 13. Visualization of Notch Signaling Oscillation in Cells and Tissues Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama 14. Proteomic Analysis of the Notch Interactome K. G. Guruharsha, Kazuya Hori, Robert A. Obar, and Spyros Artavanis-Tsakonas 15. Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands Pat Whiteman, Christina Redfield and Penny A. Handford 16. Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry Shinako Kakuda and Robert S. Haltiwanger 17. Assay to Probe Proteolytic Processing of Notch by Gamma-secretase Lutgarde Serneels, Ina Tesseur and Bart De Strooper 18. Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift Assay Kelly L. Arnett and Stephen C. Blacklow 19. Identifying Direct Notch Transcriptional Targets using the GSI-washout Assay Will Bailis, Yumi Yashiro-Ohtani and Warren S. Pear 20. Probing the Epigenetic Status at Notch Target Genes Tilman Borggrefe and Robert Liefke 21. Notch-ligand Binding Assays in Drosophila Cells Aiguo Xu and Kenneth D. Irvine 22. Modeling Notch Signaling: A Practical Tutorial Pau Formosa-Jordan and David Sprinzak 23. Small Molecules that Inhibit Notch Signaling Gerdien E. De Kloe and Bart De Strooper 24. Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling Wendy R. Gordon and Jon C. Aster 25. Application of Anti-ligand Antibodies to Inhibit Notch Signaling Jun-ichiro Koga and Masanori Aikawa


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