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Protocols in in-vitro hepatocyte research

Protocols in in-vitro hepatocyte research (Loan 1 times)

Material type
Personal Author
Vinken, Mathieu. Rogiers, Vera.
Title Statement
Protocols in in-vitro hepatocyte research / edited by Mathieu Vinken, Vera Rogiers.
Publication, Distribution, etc
New York :   Humana Press :   Springer,   2015.  
Physical Medium
xvi, 406 p. : ill. (some col.) ; 27 cm.
Series Statement
Springer protocols
9781493920730 1493920731
Bibliography, Etc. Note
Includes bibliographical references and index.
Subject Added Entry-Topical Term
Liver cells --Laboratory manuals. Hepatocytes --cytology --Laboratory Manuals. Cell Culture Techniques --methods --Laboratory Manuals.
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020 ▼a 9781493920730
020 ▼a 1493920731
035 ▼a (KERIS)REF000017690167
040 ▼a BTCTA ▼b eng ▼e rda ▼c BTCTA ▼d YDXCP ▼d OCLCQ ▼d IDL ▼d OCLCO ▼d KUB ▼d 211009
050 4 ▼a QP185 ▼b .P76 2015
082 0 4 ▼a 612.35 ▼2 23
084 ▼a 612.35 ▼2 DDCK
090 ▼a 612.35 ▼b P967
245 0 0 ▼a Protocols in in-vitro hepatocyte research / ▼c edited by Mathieu Vinken, Vera Rogiers.
260 ▼a New York : ▼b Humana Press : ▼b Springer, ▼c 2015.
300 ▼a xvi, 406 p. : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Springer protocols
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 1250
504 ▼a Includes bibliographical references and index.
650 0 ▼a Liver cells ▼v Laboratory manuals.
650 1 2 ▼a Hepatocytes ▼x cytology ▼v Laboratory Manuals.
650 2 2 ▼a Cell Culture Techniques ▼x methods ▼v Laboratory Manuals.
700 1 ▼a Vinken, Mathieu.
700 1 ▼a Rogiers, Vera.
830 0 ▼a Springer protocols.
830 0 ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v v. 1250.
945 ▼a KLPA

Holdings Information

No. Location Call Number Accession No. Availability Due Date Make a Reservation Service
No. 1 Location Science & Engineering Library/Sci-Info(Stacks2)/ Call Number 612.35 P967 Accession No. 121232451 Availability Available Due Date Make a Reservation Service B M

Contents information

Table of Contents

Part I: In Vitro Models of the Hepatocyte Life Cycle1. Isolation And Culture Of Mouse Hepatocytes: Gender-Specific Gene Expression Responses To Chemical Treatments Lyndsey Houseman, Mina Edwards, Ian R. Phillips, and Elizabeth A. Shephard 2. Cryopreservation of Hepatocytes Nicola J. Hewitt and Albert P. Li 3. Culture Conditions Promoting Hepatocyte Proliferation And Cell Cycle Synchronization Anne Corlu and Pascal Loyer 4. Immortalized Human Hepatic Cell Lines For In Vitro Testing And Research Purposes Eva Ramboer, Tamara Vanhaecke, Vera Rogiers, and Mathieu Vinken 5. Culture and Functional Characterization Of Human Hepatoma Hepg2 Cells Maria Teresa Donato, Laia Tolosa, and Maria Jose Gomez-Lechon 6. Establishment And Characterization Of An In Vitro Model Of Fas-Mediated Hepatocyte Cell Death Mathieu Vinken, Michael Maes, Sara Crespo Yanguas, Joost Willebrords, Tamara Vanhaecke, and Vera Rogiers 7. Serum-Free Directed Differentiation Of Human Embryonic Stem Cells To Hepatocytes Kate Cameron, Baltasar Lucendo-Villarin, Dagmara Szkolnicka, and David C. Hay 8. Human Skin-Derived Precursor Cells: Isolation, Expansion And Hepatic Differentiation Joery De Kock, Robim M. Rodrigues, Karolien Buyl, Tamara Vanhaecke, Vera Rogiers 9. Generation Of Hepatocytes From Pluripotent Stem Cells For Drug Screening and Developmental Modeling Richard L. Gieseck III, Ludovic Vallier, and Nicholas R.F. Hannan 10. Differentiation-Promoting Medium Additives For Hepatocyte Cultivation and Cryopreservation Varvara Gouliarmou, Olavi Pelkonen, and Sandra Coecke11. Co-Culture and Long-Term Maintenance Of Hepatocytes Merav Cohen, Gahl Levy, and Yaakov Nahmias 12. Primary Hepatocytes In Sandwich Culture Janneke Keemink, Marlies Oorts, and Pieter Annaert 13. Establishing Liver Bioreactors For In Vitro Research Sofia P. Rebelo, Rita Costa, Marcos F.Q. Sousa, Catarina Brito, and Paula M. Alves 14. Epigenetic Modifications As Anti-Dedifferentiation Strategy For Primary Hepatocytes In Culture Jennifer Bolleyn, Joanna Fraczek, Vera Rogiers, and Tamara Vanhaecke 15. Transfection Of Primary Hepatocytes With Liver-Enriched Transcription Factors Using Adenoviral Vectors Marta Benet, Ramiro Jover, and Roque Bort Part II: In Vitro Methods to Probe Hepatocyte Functionality and Toxicity 16. Transcriptomics Of Hepatocytes Treated With Toxicants For Investigating Molecular Mechanisms Underlying Hepatotoxicity Vaibhav Shinde, Regina Stober, Harshal Nemade, Isaia Sotiriadou, Jurgen Hescheler, JanHengstler, and Agapios Sachinidis 17. Global Microrna Analysis In Primary Hepatocyte Cultures Julian Krauskopf, Almudena Espin Perez, Jos C. Kleinjans, and Theo M. de Kok 18. Mass Spectrometry-Based Proteomics For Relative Protein Quantification And Biomarker Identification In Primary Human Hepatocytes Lisa Dietz and Albert Sickmann 19. Targeted Metabolomics For Homocysteine-Related Metabolites In Primary Hepatocytes Irena Selicharova and Marek Korinek 20. Measurement Of Cytochrome P450 Enzyme Induction And Inhibition In Human Hepatoma Cells Robim M. Rodrigues, Joery De Kock, Tatyana Doktorova, Vera Rogiers, and Tamara Vanhaecke 21. Analysis Of Sinusoidal Drug Uptake Transporter Activities In Primary Human Hepatocytes Marc Le Vee, Elodie Jouan, Claire Denizot, Yannick Parmentier, and Olivier Fardel 22. Measurement Of Albumin Secretion As Functionality Test In Primary Hepatocyte Cultures Karolien Buyl, Joery De Kock, Jennifer Bolleyn, Vera Rogiers, and Tamara Vanhaecke 23. Measurement Of Blood Coagulation Factor Synthesis In Cultures Of Human Hepatocytes Stefan Heinz and Joris Braspenning 24. Functionality Testing Of Primary Hepatocytes In Culture By Measuring Urea Synthesis Jennifer Bolleyn, Vera Rogiers, and Tamara Vanhaecke 25. Assay Of Bile Acid Conjugation And Excretion In Human Hepatocytes Helene Johansson and Ewa C.S. Ell

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