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Practical flow cytometry in haematology diagnosis

Practical flow cytometry in haematology diagnosis (5회 대출)

자료유형
단행본
개인저자
Leach, Richard M., MD. Drummond, Mark, PhD. Doig, Allyson.
서명 / 저자사항
Practical flow cytometry in haematology diagnosis / Mike Leach, Mark Drummond, Allyson Doig.
발행사항
Chichester, West Sussex, UK :   Wiley-Blackwell,   2013.  
형태사항
viii, 248 p. : ill.(chiefly col.) ; 26 cm.
ISBN
9780470671207 (hardback : alk. paper) 9781118487952 (Mobi) 9781118487969 (obook) 9781118487976 (epub) 9781118487990 (ePDF/ebook)
요약
"This book acts as a clinical manual for the diagnostician who cannot turn to reference books when the morphology or immunophenotype are atypical. This volume presents a logical practical approach to the diagnosis of blood disorders, both neoplastic and reactive, and other diagnostic applications of flow cytometry in non-neoplastic haematology diagnosis. Illustrations are provided throughout with worked examples"--Provided by publisher.
내용주기
Introduction -- Principles of Flow Cytometry -- Limitations -- Normal blood and bone marrow populations -- Acute Leukaemia -- Chronic lymphoid leukaemias and exfoliating lymphoma -- Myelodysplastic Syndromes and Myeloproliferative Neoplasms -- Disorders of plasma cells -- Minimal residual disease -- Red cells, leucocytes, and platelets -- Reactive and non-neoplastic phenomena.
서지주기
Includes bibliographical references and index.
일반주제명
Hematologic Diseases -- diagnosis. Hematologic Neoplasms -- diagnosis. Flow Cytometry -- methods. Hematology -- methods.
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001 000045754989
005 20130618171841
008 120726s2013 enka b 001 0 eng
010 ▼a 2012029836
020 ▼a 9780470671207 (hardback : alk. paper)
020 ▼a 9781118487952 (Mobi)
020 ▼a 9781118487969 (obook)
020 ▼a 9781118487976 (epub)
020 ▼a 9781118487990 (ePDF/ebook)
035 ▼a (KERIS)REF000016894442
040 ▼a DNLM/DLC ▼c DLC ▼d 211009
042 ▼a pcc
060 1 0 ▼a WH 120
082 0 0 ▼a 616.1/5075 ▼2 23
084 ▼a 616.15075 ▼2 DDCK
090 ▼a 616.15075 ▼b L434p
100 1 ▼a Leach, Richard M., ▼c MD.
245 1 0 ▼a Practical flow cytometry in haematology diagnosis / ▼c Mike Leach, Mark Drummond, Allyson Doig.
260 ▼a Chichester, West Sussex, UK : ▼b Wiley-Blackwell, ▼c 2013.
300 ▼a viii, 248 p. : ▼b ill.(chiefly col.) ; ▼c 26 cm.
504 ▼a Includes bibliographical references and index.
505 0 ▼a Introduction -- Principles of Flow Cytometry -- Limitations -- Normal blood and bone marrow populations -- Acute Leukaemia -- Chronic lymphoid leukaemias and exfoliating lymphoma -- Myelodysplastic Syndromes and Myeloproliferative Neoplasms -- Disorders of plasma cells -- Minimal residual disease -- Red cells, leucocytes, and platelets -- Reactive and non-neoplastic phenomena.
520 ▼a "This book acts as a clinical manual for the diagnostician who cannot turn to reference books when the morphology or immunophenotype are atypical. This volume presents a logical practical approach to the diagnosis of blood disorders, both neoplastic and reactive, and other diagnostic applications of flow cytometry in non-neoplastic haematology diagnosis. Illustrations are provided throughout with worked examples"--Provided by publisher.
650 1 2 ▼a Hematologic Diseases ▼x diagnosis.
650 1 2 ▼a Hematologic Neoplasms ▼x diagnosis.
650 2 2 ▼a Flow Cytometry ▼x methods.
650 2 2 ▼a Hematology ▼x methods.
700 1 ▼a Drummond, Mark, ▼c PhD.
700 1 ▼a Doig, Allyson.

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 의학도서관/자료실(3층)/ 청구기호 616.15075 L434p 등록번호 131046333 도서상태 대출가능 반납예정일 예약 서비스 B
No. 2 소장처 의학도서관/자료실(3층)/ 청구기호 616.15075 L434p 등록번호 131052271 도서상태 대출가능 반납예정일 예약 서비스 B
No. 3 소장처 의학도서관/자료실(3층)/ 청구기호 616.15075 L434p 등록번호 131052272 도서상태 대출가능 반납예정일 예약 서비스 B

컨텐츠정보

목차

Preface, vii

Acknowledgements, viii

1 Introduction, 1

References, 2

2 Principles of Flow Cytometry, 3

Introduction, 3

Sample preparation, 3

The flow cytometer, 4

The fluidic system, 4

The optical system, 5

The electronic system, 9

Data display, 9

Acknowledgements, 18

References, 19

3 Limitations, 20

Introduction, 20

Clinical context issues, 20

Sampling issues, 20

Technical issues, 25

Interpretation issues, 28

Conclusion, 29

References, 29

4 Normal Blood and Bone Marrow Populations, 31

Normal stem and precursor cell populations, 31

References, 41

5 Acute Leukaemia, 43

Introduction, 43

Identification of neoplastic precursor cells, 45

Lineage assignment of acute leukaemia, 52

Acute myeloid leukaemia, 54

Precursor lymphoid neoplasms (acute lymphoblastic leukaemia/lymphoma), 77

Examination of cerebro-spinal fluid and serous effusions, 88

Identification of genetic abnormalities by flow, 92

Conclusion, 93

References, 93

6 Chronic Lymphoid Leukaemias and Exfoliating Lymphoma, 100

Normal peripheral blood lymphoid populations, 100

Identification of clonal lymphoid populations, 101

Identification of clonal B-cell disorders, 101

CD5 positive B-cell lymphoproliferative disorders, 102

CD10 positive B-cell lymphoproliferative disorders, 116

B-cell disorders identified using an extended B-cell panel, 121

Identification of clonal T-cell disorders, 125

CD4 positive T-cell disorders, 128

CD8 positive T-cell disorders, 136

CD4/CD8 positive T-cell disorders, 141

CD4/8 negative disorders, 141

Flow cytometric assessment of serous effusions, 146

Flow cytometric assessment of cerebrospinal fluid, 146

Summary, 149

References, 149

7 Myelodysplastic Syndromes and Myeloproliferative Neoplasms, 152

Introduction, 152

Myelodysplastic syndromes, 152

Myelodysplastic/myeloproliferative neoplasms, 163

Chronic myeloid leukaemia, 165

The BCR-ABL negative myeloproliferative neoplasms, 166

Mastocytosis, 167

Summary, 170

References, 170

8 Disorders of Plasma Cells, 173

Plasma cell disorders, 173

Current diagnostic criteria, 173

Plasma cell morphology, 175

The applications of immunophenotyping to plasma cell disorders, 178

References, 183

9 Minimal Residual Disease, 184

Introduction, 184

Minimal residual disease analysis in the management of acute lymphoblastic leukaemia, 187

Conclusions, 199

Acknowledgements, 200

References, 200

10 Red Cells, Leucocytes and Platelets, 202

Paroxysmal nocturnal haemoglobinuria, 202

Red cell membrane disorders, 208

Foetal maternal haemorrhage, 211

Lymphocyte subset analysis

and immunodeficiency, 214

Haemopoietic stem cell enumeration, 214

Granulocyte disorders, 215

Platelet disorders, 219

References, 221

11 Reactive and Non-neoplastic Phenomena, 225

Peripheral blood, 225

Bone marrow, 231

Pleural and pericardial fluids, 236

Bronchoalveolar lavage specimens, 238

Cerebrospinal fluid, 238

References, 240

Index, 243


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