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Cancer cell culture : methods and protocols 2nd ed

Cancer cell culture : methods and protocols 2nd ed (6회 대출)

자료유형
단행본
개인저자
Cree, Ian A.
서명 / 저자사항
Cancer cell culture : methods and protocols / edited by Ian A. Cree.
판사항
2nd ed.
발행사항
Totowa, N.J. :   Humana Press,   c2011.  
형태사항
xiv, 502 p. : ill. (some col.) ; 27 cm.
총서사항
Methods in molecular biology,1064-3745 ; v. 731
ISBN
9781617790799 (alk. paper) 1617790796 (alk. paper) 9781617790805 (ebk.)
내용주기
Xenotransplantation of breast cancers -- Feeder layers: Co-culture with nonneoplastic cells -- Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3h]-thymidine incorporation assay -- Transfection and DNA-mediated gene transfer -- Analysis of drug interactions -- Development of rituximab-resistant B-NHL clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy -- Proteomic evaluation of cancer cells: Identification of cell surface proteins -- Measuring gene expression from cell cultures by quantitative reverse-transcriptase polymerase chain reaction -- Expression analysis of homeobox genes in leukemia/lymphoma cell lines -- Flow cytometric DNA analysis of human cancers and cell lines -- Angiogenesis assays -- Cell migration and invasion assays -- Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR -- Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay -- Purification of annexin V and its use in the detection of apoptotic cells -- Real-time cytotoxicity assays -- Differential staining cytotoxicity assay: A review -- Cell sensitivity assays: The ATP-based tumor chemosensitivity assay -- Cell sensitivity assays: The MTT assay -- Cellular chemosensitivity assays: An overview -- Isolation of endothelial cells from human tumors -- Isolation of inflammatory cells from human tumours -- Establishment and culture of leukemia-lymphoma cell lines -- Isolation and culture of ovarian cancer cells and cell lines -- Isolation and culture of squamous cell carcinoma lines -- Isolation and culture of melanoma and naevus cells and cell lines -- Isolation and culture of colon cancer cells and cell lines -- Generation of lung cancer cell line variants by drug selection or cloning -- Quality assurance and good laboratory practice -- Elimination of mycoplasmas from infected cell lines using antibiotics -- Detecting mycoplasma contamination in cell cultures by polymerase chain reaction -- Cell culture contamination -- Cytogenetic analysis of cancer cell lines -- Online verification of human cell line identity by STRDNA typing -- Characterization and authentication of cancer cell lines: An overview -- Storage of cell lines -- Principles of cancer cell culture -- Cancer biology.
서지주기
Includes bibliographical references and index.
일반주제명
Cancer cells -- Laboratory manuals. Tumor Cells, Cultured -- Laboratory manuals. Laboratory Techniques and Procedures -- Laboratory manuals.
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010 ▼a 2011926687
020 ▼a 9781617790799 (alk. paper)
020 ▼a 1617790796 (alk. paper)
020 ▼a 9781617790805 (ebk.)
035 ▼a (KERIS)REF000016450244
040 ▼a YDXCP ▼c YDXCP ▼d ZCH ▼d CUS ▼d GZM ▼d CDX ▼d SCB ▼d DLC ▼d 211009
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082 0 0 ▼a 616.99/40078 ▼2 22
084 ▼a 616.9940078 ▼2 DDCK
090 ▼a 616.9940078 ▼b C215-2
245 0 0 ▼a Cancer cell culture : ▼b methods and protocols / ▼c edited by Ian A. Cree.
250 ▼a 2nd ed.
260 ▼a Totowa, N.J. : ▼b Humana Press, ▼c c2011.
300 ▼a xiv, 502 p. : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v v. 731
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
505 0 ▼a Xenotransplantation of breast cancers -- Feeder layers: Co-culture with nonneoplastic cells -- Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3h]-thymidine incorporation assay -- Transfection and DNA-mediated gene transfer -- Analysis of drug interactions -- Development of rituximab-resistant B-NHL clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy -- Proteomic evaluation of cancer cells: Identification of cell surface proteins -- Measuring gene expression from cell cultures by quantitative reverse-transcriptase polymerase chain reaction -- Expression analysis of homeobox genes in leukemia/lymphoma cell lines -- Flow cytometric DNA analysis of human cancers and cell lines -- Angiogenesis assays -- Cell migration and invasion assays -- Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR -- Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay -- Purification of annexin V and its use in the detection of apoptotic cells -- Real-time cytotoxicity assays -- Differential staining cytotoxicity assay: A review -- Cell sensitivity assays: The ATP-based tumor chemosensitivity assay -- Cell sensitivity assays: The MTT assay -- Cellular chemosensitivity assays: An overview -- Isolation of endothelial cells from human tumors -- Isolation of inflammatory cells from human tumours -- Establishment and culture of leukemia-lymphoma cell lines -- Isolation and culture of ovarian cancer cells and cell lines -- Isolation and culture of squamous cell carcinoma lines -- Isolation and culture of melanoma and naevus cells and cell lines -- Isolation and culture of colon cancer cells and cell lines -- Generation of lung cancer cell line variants by drug selection or cloning -- Quality assurance and good laboratory practice -- Elimination of mycoplasmas from infected cell lines using antibiotics -- Detecting mycoplasma contamination in cell cultures by polymerase chain reaction -- Cell culture contamination -- Cytogenetic analysis of cancer cell lines -- Online verification of human cell line identity by STRDNA typing -- Characterization and authentication of cancer cell lines: An overview -- Storage of cell lines -- Principles of cancer cell culture -- Cancer biology.
650 0 ▼a Cancer cells ▼v Laboratory manuals.
650 1 2 ▼a Tumor Cells, Cultured ▼v Laboratory manuals.
650 2 2 ▼a Laboratory Techniques and Procedures ▼v Laboratory manuals.
700 1 ▼a Cree, Ian A.
830 0 ▼a Springer protocols.
830 0 ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v v. 731.
945 ▼a KLPA

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 616.9940078 C215-2 등록번호 121213740 도서상태 대출가능 반납예정일 예약 서비스 B M
No. 2 소장처 의학도서관/자료실(3층)/ 청구기호 616.9940078 C215-2 등록번호 131042261 도서상태 대출가능 반납예정일 예약 서비스 B
No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 616.9940078 C215-2 등록번호 121213740 도서상태 대출가능 반납예정일 예약 서비스 B M
No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 의학도서관/자료실(3층)/ 청구기호 616.9940078 C215-2 등록번호 131042261 도서상태 대출가능 반납예정일 예약 서비스 B

컨텐츠정보

목차

1. Cancer Biology Ian A. Cree 2. Principles of Cancer Cell Culture Ian A. Cree 3. Storage of Cell Lines Katharine A. Parker 4. Characterization and Authentication of Cancer Cell Lines: An Overview Yvonne A. Reid 5. Online Verification of Human Cell Line Identity by STR DNA Typing Wilhelm G. Dirks and Hans G. Drexler 6. Cytogenetic Analysis of Cancer Cell Lines Roderick A. F. MacLeod and Hans G. Drexler 7. Cell Culture Contamination Glyn N. Stacey 8. Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction Cord C. Uphoff and Hans G. Drexler 9. Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics Cord C. Uphoff and Hans G. Drexler 10. Quality Assurance and Good Laboratory Practice Louise A. Knight and Ian A. Cree 11. Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning Laura Breen, Joanne Keenan, and Martin Clynes 12. Isolation and Culture of Colon Cancer Cells and Cell Lines Sharon Glaysher and Ian A. Cree 13. Isolation and Culture of Melanoma and Naevus Cells and Cell Lines Julia K. Soo, Alastair D. MacKenzie Ross, and Dorothy C. Bennett 14. Isolation and Culture of Squamous Cell Carcinomalines Karin J. Purdie, Celine Pourreyron, and Andrew P. South 15. Isolation and Culture of Ovarian Cancer Cells and Cell Lines Christian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween, Jutta A. Kurbacher, Ralf Reichelt, and Petra N. Arenz 16. Establishment and Culture of Leukemia-Lymphoma Cell Lines Hans G. Drexler 17. Isolation of Inflammatory Cells from Human Tumors Marta Polak 18. Isolation of Endothelial Cells from Human Tumors Elisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau, Roland S. Croner, and Michael Sturzl 19. Cellular Chemosensitivity Assays: An Overview Venil N. Sumantran 20. Cell Sensitivity Assays: The MTT Assay J. van Meerlo, G.J.L. Kaspers, and J.Cloos 21. Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay Sharon Glaysher and Ian A. Cree 22. The Differential Staining Cytotoxicity (DiSC) Assay: A Review Larry M. Weisenthal 23. Real-time Cytotoxicity Assays Donald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz, and Jonathan M. Cooper 24. FPurification of Annexin V and its use in the Detection of Apoptotic Cells Katy M. Coxon, James Duggan, M. Francesca Cordeiro, and Stephen E. Moss 25. Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay Janet M. Hartley, Victoria J. Spanswick, and John A. Hartley 26. Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR Bjorn Schneider, Hans G. Drexler, and Roderick A.F. MacLeod 27. Cell Migration and Invasion Assays K.A. Moutasim, M.L. Nystrom, and G.J. Thomas 28. Angiogenesis Assays V. Poulaki 29. Flow Cytometric DNA Analysis of Human Cancers and Cell Lines Sarah A. Krueger and George D. Wilson 30. Expression Analysis of Homeobox Genes in Leukemia/Lymphoma Cell Lines Stefan Nagel and Hans G. Drexler 31. Measuring Gene Expression from Cell Cultures by Quantitative Reverse-Transciptase Polymerase Chain Reaction Sharon Glaysher, Francis G. Gabriel, and Ian A. Cree 32. Proteomic Evaluation of Cancer Cells - Identification of Cell Surface Proteins Samantha Larkin and Claire Hastie 33. Development of Rituximab-resistant B-NHL Clones: An In Vitro Model for Studying Tumor Resistance to Monoclonal Antibody-mediated Immunotherapy Ali R. Jazirehi and Bejamin Bonavida 34. High Throughput Screening of Natural Products for Cancer Therapy: A Review Alan L. Harvey and Ian A. Cree 35. Analysis of Drug Interactions Irene V. Bijnsdorp, Elisa Giovannetti, and Godefridus J. Peters 36. Transfection and DNA-mediated Gene Transfer Davide Zecchin and Federica Di Nicolantonio 37. Drug Design and Testing:


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