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Clusterin as a Potential Regulator for Cell Proliferation and Differentiation

Clusterin as a Potential Regulator for Cell Proliferation and Differentiation

자료유형
학위논문
개인저자
강상욱
서명 / 저자사항
Clusterin as a Potential Regulator for Cell Proliferation and Differentiation / Sang-Wook Kang.
발행사항
서울 :   고려대학교 대학원 ,   2003.  
형태사항
ⅸ, 117 p. : 삽도 ; 26 cm.
학위논문주기
학위논문(박사) -- 고려대학교 대학원 생명공학과 의약생명공학전공 2002
학과코드
0510   6YD48   65  
서지주기
참고문헌 : p. 100-109
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100 1 ▼a 강상욱
245 1 0 ▼a Clusterin as a Potential Regulator for Cell Proliferation and Differentiation / ▼d Sang-Wook Kang.
260 ▼a 서울 : ▼b 고려대학교 대학원 , ▼c 2003.
300 ▼a ⅸ, 117 p. : ▼b 삽도 ; ▼c 26 cm.
502 1 ▼a 학위논문(박사) -- ▼b 고려대학교 대학원 ▼c 생명공학과 의약생명공학전공 ▼d 2002
504 ▼a 참고문헌 : p. 100-109

전자정보

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Clusterin as a Potential Regulator for Cell Proliferation and Differentiation
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No. 1 소장처 과학도서관/학위논문서고/ 청구기호 0510 6YD48 65 등록번호 123022934 도서상태 대출가능 반납예정일 예약 서비스 B M
No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 세종학술정보원/학위논문실/ 청구기호 0510 6YD48 65 등록번호 153038434 도서상태 대출가능 반납예정일 예약 서비스

컨텐츠정보

초록

Clusterin is widely up-regulated in many tissues in association with various patho-physiological conditions, its upregulation being implicated in various biological processes such as cell proliferation, differentiation, and apoptotic cell death. However, it remains to be further elucidated about how clusterin could exert its function in those opposing processes, since proliferation, differentiation and apoptosis are contradictory in cellular events. Clusterin is known to bind to many molecules including heparin, beta-amyloid, prion peptide, paraoxnase, immunoglobulin, complement components, and lipids. The binding ability of clusterin to other molecules and its involvement in various biological processes led us to hypothesize that its function might be modulated by interacting with specific effecter molecules which are involved in opposing cellular events. Thus, in this dissertation, we investigated the regulation of clusterin and its interacting molecules involved in the proliferation of primary astrocytes as well as in the neuronal differentiation of PC12 cells.
Clusterin expression is increased in the reactive astrocytes following experimental brain injuries as well as in disease states such as inflammation and neurodegeneration, although its function has not been clearly elucidated. Thus, whether or not clusterin could have any effect on proliferation of reactive astrocytes was examined. Primary astorcytes transfected with clusterin cDNA (pcDNA3-CLU) highly expressed clusterin, while the basal low level of clusterin was detected in the cells transfected with the control plasmid (pcDNA3). Transient clusterin over-expression enhanced incorporation of 3[H]-thymidine into DNA synthesis of primary astrocytes by 50 % compared to the control vector transfected cells at 24 hours after transfection of clusterin cDNA. Proliferation property of clusterin in astrocytes was further confirmed by PCNA immunostaining and western blot analysis. Its proliferation activity is cert

목차

Table of Contents = ⅰ
List of Tables = ⅰ
List of Figures = ⅱ
List of Abbreviations = ⅲ
Abstract = ⅴ
Chapter Ⅰ. Clusterin stimulates proliferation of primary-cultured rat astrocytes = 1
 1. Abstract = 2
 2. Introduction = 4
 3. Materials and Methods = 7
 4. Results = 14
 5. Discussion = 33
 6. References = 38
Chapter Ⅱ. Clusterin interacts with SCLIP and promotes neurite outgrowth of PC12 cells = 49
 1. Abstract = 50
 2. Introduction = 53
 3. Materials and Methods = 57
 4. Results = 64
 5. Discussion = 91
 6. References = 100
Abstract in Korean = 110
Acknowledgement = 115