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Peptide hormone action : a practical approach

Peptide hormone action : a practical approach

자료유형
단행본
개인저자
Siddle, K. Hutton, J. C.
서명 / 저자사항
Peptide hormone action : a practical approach / edited by K. Siddle and J.C. Hutton.
발행사항
Oxford ;   New York :   IRL Press at Oxford University Press,   c1990.  
형태사항
xix, 256 p. : ill. ; 25 cm.
총서사항
The Practical approach series
ISBN
0199630704 (hard) : 0199630712 (pbk.) : 0199630690 (pbk.) : 0199630720 (2 v. set : spiral hardback) 0199630739 (2 v. set : papercover)
일반주기
Companion vol. to: Peptide hormone secretion.  
서지주기
Includes bibliographical references and index.
일반주제명
Peptide hormones --Research --Methodology. Peptide hormones --Receptors --Research --Methodology. Second messengers (Biochemistry) --Research --Methodology. Hormones --analysis. Hormones --physiology. Peptides --analysis. Peptides --physiology.
비통제주제어
Polypeptides,,
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019 ▼a 24034671 ▼a 25592160
020 ▼a 0199630704 (hard) : ▼c $78.00
020 ▼a 0199630712 (pbk.) : ▼c $50.00
020 ▼a 0199630690 (pbk.) :
020 ▼a 0199630720 (2 v. set : spiral hardback)
020 ▼a 0199630739 (2 v. set : papercover)
040 ▼a DNLM/DLC ▼c DLC ▼d UKM ▼d NLM ▼d AGL
049 ▼a ACSL ▼l 421116941
050 0 0 ▼a QP572.P4 ▼b P43 1990
060 0 ▼a WK 185 ▼b P4233 1990
069 0 ▼a 9005475
070 0 ▼a QP572.P4P43 ▼b 1990
072 0 ▼a X300
082 0 0 ▼a 612.4/05 ▼2 20
090 ▼a 612.405 ▼b P424
245 0 0 ▼a Peptide hormone action : ▼b a practical approach / ▼c edited by K. Siddle and J.C. Hutton.
260 ▼a Oxford ; ▼a New York : ▼b IRL Press at Oxford University Press, ▼c c1990.
300 ▼a xix, 256 p. : ▼b ill. ; ▼c 25 cm.
440 4 ▼a The Practical approach series
500 ▼a Companion vol. to: Peptide hormone secretion.
504 ▼a Includes bibliographical references and index.
650 0 ▼a Peptide hormones ▼x Research ▼x Methodology.
650 0 ▼a Peptide hormones ▼x Receptors ▼x Research ▼x Methodology.
650 0 ▼a Second messengers (Biochemistry) ▼x Research ▼x Methodology.
650 2 ▼a Hormones ▼x analysis.
650 2 ▼a Hormones ▼x physiology.
650 2 ▼a Peptides ▼x analysis.
650 2 ▼a Peptides ▼x physiology.
653 ▼a Polypeptides
700 1 ▼a Siddle, K.
700 1 ▼a Hutton, J. C.
730 0 ▼a Peptide hormone secretion.

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 612.405 P424 등록번호 421116941 도서상태 대출가능 반납예정일 예약 서비스 B M
No. 2 소장처 의학도서관/자료실(3층)/ 청구기호 612.405 P424 등록번호 131038182 도서상태 대출가능 반납예정일 예약 서비스 B
No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 612.405 P424 등록번호 421116941 도서상태 대출가능 반납예정일 예약 서비스 B M
No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 의학도서관/자료실(3층)/ 청구기호 612.405 P424 등록번호 131038182 도서상태 대출가능 반납예정일 예약 서비스 B

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CONTENTS
Contributors = xv
Abbreviations = xvii
1. Peptide hormone receptors / S. Gammeltoft = 1
 1. Introduction = 1
 2. Labelling of peptide hormones = 2
 3. Iodination procedures = 2
  Chloramine-T method = 2
  Iodogen method = 4
  Bolton-Hunter method = 5
  Other iodination methods = 5
  Heterogeneity of iodinated peptides = 6
  Validation of tracers = 7
  Determination of specific activity of the labelled peptide = 8
 4. Receptor-binding of peptides = 8
  $${}^{125}I$$-labelled peptide = 9
  Receptor preparation = 9
  Preparation of isolated cells = 10
  Preparation of plasma membranes = 12
  Preparation of solubilized receptors = 13
  Conditions of binding assay = 15
  Separation of bound and free tracer = 16
  Cell receptor-binding assay = 17
  Plasma membrane receptor-binding assay = 18
  Solubilized receptor-binding assay = 19
 5. Binding properties of peptide hormone receptors = 20
  Receptor-binding theory = 20
  Transient-state binding kinetics = 21
  Steady-state binding kinetics = 23
  Peptide specificity = 24
 6. Analysis of radioligand binding = 25
  Graphical representations = 26
  Computer programs = 31
 7. Receptor-mediated endocytosis and degradation of peptide hormones = 32
  Surface-binding and internalization assay = 32
  Recycling of receptors = 34
  Proteolytic degradation of peptide hormones = 36
  Trichloroacetic acid precipitation = 37
  Immunoprecipitation assay = 38
  Gel filtration chromatographic assay = 39
 Acknowledgements = 40
 References = 41
2. Receptor characterization / J. L. Treadway ; J. E. Pessin = 43
 1. Introduction = 43
 2. Microsomal membrane preparation = 43
  Human placenta = 44
  Liver = 45
  Adipose tissue = 46
  Cultured cells = 46
 3. Solubilization = 47
  Membranes = 47
  Tissue = 48
  Preservation of in vivo phosphorylation state = 49
 4. Partial purification of receptors = 49
  Immobilized lectins = 49
  Preparative gel fitration chromatography = 51
 5. Homogeneous purification of receptors = 54
  Preparation of affinity resin = 55
  Purification procedure = 57
  Concentration of purified receptors = 60
 6. Preparing insulin and IGF-I receptor heterodimers = 61
  Solubilized receptors = 62
  Membrane receptors = 63
 7. Hydrodynamic properties = 64
  Analytic gel filtration chromatography = 64
  Sucrose gradient centrifugation = 65
 8. Affinity labelling = 66
  Intact cells = 67
  Membranes = 68
  Solubilized receptors = 69
 References = 70
3. Cyclic nucleotides / T. Lakey ; N. Pyne ; G. Murphy = 73
 1. General introduction = 73
 2. Measurement of cyclic nucleotides = 73
  Introduction = 73
  Incubation conditions = 74
  Termination of incubation = 75
  Preparation of samples for cyclic nucleotide determination = 75
  Assay of cyclic nucleotide levels by radioimmunoassay = 78
  Assay of cyclic AMP by binding protein technique = 81
 3. Measurement of adenylate cyclase activity = 84
  Introduction = 84
  Assay of adenylate cyclase using $${}^{32}P$$-ATP = 84
  Non-labelled incubation for assay of adenylate cyclase = 92
  Use of cholera and pertussis toxins = 93
  Use of forskolin to assay $$G_i$$ function = 95
 4. Measurement of guanylate cyclase activity = 96
  Introduction = 96
  Assay overview = 96
  Enzyme preparation = 97
  Activators and inhibitors = 98
  Incubation conditions = 98
  Isolation of reaction product = 100
 5. Assay of cyclic nucleotide phosphodiesterase activity = 101
  Introduction = 101
  Overview of assay = 101
  Assay procedure = 102
  Preparation of the enzyme sample = 105
  Further techniques for investigation of phosphodiesterase = 108
 6. Measurement of cyclic-AMP-dependent protein kinase activity = 110
  Introduction = 110
  Assay methodology = 110
  Assay of the inhibitor protein = 111
 Acknowledgements = 112
 References = 112
4. Cytoplasmic free calcium : measurement and manipulation in living cells / M. B. Hallet ; R. L. Dormer ; A. K. Campbell = 115
 1. A role for cytosolic C$$a^{2+}$$ in cell activation and injury = 115
 2. Principles of measurement of cytosolic C$$a^{2+}$$ = 116
 3. Fluorescent chelator indicators = 117
  Incorporation of indicators into cells = 118
  Calculation of cytoplasmic C$$\a^{2+}$$ from fluorescent signals = 121
  Problems of interpreting fluorescent signals = 122
  Instrumentation = 127
 4. Photoproteins = 129
  Preparation of reagents = 131
  mRNA isolation and cloning = 133
  Incorporation into cells = 134
  Application and calibration in cells = 136
  Problems = 137
  Instrumentation = 138
 5. Other methods of measuring cytosolic C$$a^{2+}$$ = 139
  C$$a^{2+}$$-sensitive micro-electrodes = 139
  Metallochromic indicators = 139
  $${}^{19}F$$ NMR probe for free C$$a^{2+}$$ = 139
 6. C$$a^{2+}$$ flux measurement = 140
  Whole cell preparations = 140
  Procedures = 141
  Analysis of data = 144
 7. Manipulation of intracellular C$$a^{2+}$$ = 144
  C$$a^{2+}$$ ionophores = 146
  Intracellular C$$a^{2+}$$ buffers = 147
  C$$a^{2+}$$ channel blockers = 148
 8. Conclusions = 148
 Acknowledgements = 149
 References = 149
5. Inositol phosphate second messengers / C. J. Kirk ; A. J. Morris ; S. B. Shears = 151
 1. Introduction = 151
 2. Labelling protocols for investigating inositol lipids and their degradation products = 151
 3. Quenching and extraction procedures for inositol phosphates and inositol lipids = 153
  Perchloric acid quench = 154
  Trichloroacetic acid quench = 155
  Neutral quench techniques for extracting cyclic inositol phosphates = 155
  Quenching and extraction of inositol lipids = 155
 4. Ion-exchange chromatography of inositol phosphates = 157
  Analysis by gravity-fed columns = 157
  Analysis by HPLC = 158
 5. Separation techniques for the inositol lipids = 159
  Purification of phosphoinositides by affinity chromatography on neomycin-linked glass beads = 161
  Thin-layer chromatography = 162
  Deacylation of inositol lipids and the separation of glycero-phosphoinositol esters by anion-exchange chromatography = 163
  Assay of lipid phosphorus = 164
 6. Preparation of radioactive inositol phosphates = 164
  Labelling strategies = 164
  Production of inositol phosphates by chemical and enzymic degradation of inositol lipids = 166
  Preparation of metabolites of Ins(1,4,5)P₃ = 169
  Removal of salt from inositol phosphates following anion-exchange chromatography = 170
  Commercially available inositol phosphates = 171
 7. Assay of inositol phosphate phosphatases and kinases = 172
 8. Methods for determining the structure of inositol polyphosphates = 173
  Periodate oxidation, reduction, dephosphorylation, and the identification of the polyols thus produced = 173
  Separation of polyols = 176
  Use of polyol dehydrogenase to distinguish between enantiomeric pairs of polyols = 178
  Specific dephosphorylation of periodate-resistant inositol phosphates to produce periodate-sensitive compounds = 180
  Non-selective dephosphorylation of inositol phosphates and identification of the inositol monophosphates formed = 181
 Acknowledgements = 181
 References = 182
6. Lipid-related second messengers / R. V. Farese ; D. R. Cooper = 185
 1. Introduction = 185
 2. Diacylglycerol = 185
  Chemistry and molecular forms = 185
  Sources and mechanisms for induced increases = 187
  Measurement by labelling or direct assay = 190
  Biological effects = 197
  Use of phorbol esters, diacylglycerols, and other analogues = 198
 3. Protein kinase C = 202
  General aspects = 202
  Hormonal effects on enzyme activity and subcellular distribution = 202
  Standard assay = 204
  Translocation assay = 205
  Phorbol ester binding assay = 207
  Autisera and immunoassays = 208
  Phosphorylation of substrates in intact cells = 211
  Phosphoprotein markers = 213
  Use of alternative substrates in in vivo assays = 213
  Inhibitors = 213
  'Desensitization' or 'down-regulation' by phorbol esters = 213
  Purification of isoforms by hydroxylapatite chromatography = 214
 4. Arachidonate, prostaglandins, and leukotrienes = 215
  General aspects = 215
  Methods for examining products of arachidonate metabolism = 217
 References = 219
7. The insulin receptor tyrosine kinase / M. F. White = 223
 1. Introduction = 223
 2. Expression of the human insulin receptor in cultured cells = 223
  Expression plasmids used for mammalian cell transfection = 223
  Transfection of Chinese hamster ovary (CHO) cells = 225
  Selection of CHO cells expressing the human insulin receptor by fluorescence-activated cell sorting (FACS) = 226
  Insulin-binding to transfected CHO cells = 227
 3. Analysis of the phosphorylated insulin receptor in transfected CHO cells = 230
  Introduction = 230
  Phosphate labelling = 230
  Immunopurification of the insulin receptor with anti-insulin receptor antibody and separation of the phosphoproteins by SDS-PAGE = 232
  Immunopurification of the insulin receptor and other phosphotyrosyl-containing proteins with antiphosphotyrosine antibody = 235
 4. Purification of the insulin receptor on immobilized wheat germ agglutinin and in vitro autophosphorylation = 238
  Purification of the insulin receptor on immobilized wheat germ agglutinin = 238
  Autophosphorylation of the WGA-purified insulin receptor = 239
  WGA purification of the insulin receptor from phorbol ester-treated cells = 240
  WGA purification of the insulin receptor from trypsin-treated cells = 242
 5. Analysis of the phosphorylated insulin receptor = 242
  Tryptic peptide mapping of the β-subunit of the insulin receptor = 242
  Analysis of the tryptic phosphopeptide map = 243
 6. Kinase activity of the purified insulin receptor = 247
  Measuring kinase activity with synthetic peptides = 247
  Taking advantage of substrate inhibition to determine the kinase activity of the receptor stimulated in the intact cell = 248
 Acknowledgements = 249
 References = 249
Index = 251


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